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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2116-2122
DOI: https://doi.org/10.20546/ijcmas.2017.603.241


Natural Occurrence of mixed infection of Citrus Yellow Mosaic virus (CYMV) and Indian Citrus Ring Spot Virus (ICRSV) and their Detection by Duplex PCR in Sweet Orange
K.N. Gupta1*, V.K. Baranwal2 and V. Gopal3
1PC.Unit AICRP Sesame and Niger: Jawaharlal Nehru Krishi Vishwa Vidyalaya,Jabalpur 482 004, Madhya Pradesh, India
2Plant Virology Unit, Division of Plant Pathology, Indian Agricultural ResearchInstitute New Delhi –110012, India
3Citrus Research Station, Tirupati-517 502 (A.P.), India
*Corresponding author
Abstract:

Survey of Sathgudi sweet orange was done in the Nagri village of Chittor district in the A.P. state during November, 2006. Incidence of citrus yellow mosaic disease was observed up to 50% in citrus plants. A few plants also showed unusual symptoms of vein clearing and mosaic. Bacilliform and flexous rod shaped of virus particles were seen in electron microscopy in samples of the diseased citrus plants showing vein clearing and mosaic. Immunosorbent electron microscopy indicated the baciliform particles were of Citrus Yellow Mosaic Virus (CYMV) while flexuous particles were of Indian citrus ringspot virus. Since ISEM can not be used for routine detection, a duplex PCR for detection of both the viruses was standardized, using specific primers of CYMV and ICRSV providing amplification product of 537 bp and 1121bp. The sequencing of PCR product confirmed that the amplified PCR product of ICRSV and CYMV. The duplex PCR provide a useful and rapid method for molecular detection of two viruses. The technique should prove highly useful in disease surveys, nursery certification and quarantine applications.
 


Keywords: Citrus yellow mosaic virus, Citrus Ring Spot Virus and molecular detection.
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How to cite this article:

Gupta, K.N., V.K. Baranwal and Gopal, V. 2017. Natural Occurrence of mixed infection of Citrus Yellow Mosaic virus (CYMV) and Indian Citrus Ring Spot Virus (ICRSV) and their Detection by Duplex PCR in Sweet Orange.Int.J.Curr.Microbiol.App.Sci. 6(3): 2116-2122. doi: https://doi.org/10.20546/ijcmas.2017.603.241