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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 1510-1517
DOI: https://doi.org/10.20546/ijcmas.2020.907.174


Prevalence, Detection and Identification of Listeria monocytogenes in Retail Chicken Meat in Ludhiana, India by Employing conventional Isolation Techniques and Molecular Polymerase Chain Reaction (PCR) Assay
Nishchal Dutta1*, H. S. Banga2, Sidhartha Deshmukh2 and Geeta Devi Leishangthem2
1Department of Veterinary Pathology, Khalsa College of Veterinary and Animal Sciences, Amritsar-143002, Punjab, India
2Department of Veterinary Pathology, College of Veterinary Science Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

Listeria monocytogenes is one of the major food contaminants with potential to cause lethal food poisoning in both humans and animals. Among different food borne pathogens, Listeria monocytogenes has a high mortality rate and is therefore considered one of the most dangerous foodborne pathogen. The present study was aimed at finding the prevalence of Listeria monocytogenes in raw chicken meat purchased from different retail outlets and local butcher shops across the Ludhiana city. In the present study a total of 100 raw chicken meat samples were collected (80 fresh raw samples and 20 frozen chicken meat products). During this study, 100 chicken meat samples were inoculated in PALCAM selective agar for the selective isolation of L. monocytogenes and were later characterized by a combination of microscopic and biochemicaltests. Results of the study revealed that02 samples were containing L. monocytogenes, which is 02% of the total samples. These positive samples were subjected to molecular characterization using standard PCR technique to attest their presence in these meat samples. The PCR technique was found to be more specific/sensitive, reliable, precise and rapid technique to supplement the conventional methods of diagnosis of the L. monocytogenes.


Keywords: L. monocytogenes, chicken meat, PALCAM selective agar, BHI brothandPCR
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How to cite this article:

Nishchal Dutta, H. S. Banga, Sidhartha Deshmukh and Geeta Devi Leishangthem 2020. Prevalence, Detection and Identification of Listeria monocytogenes in Retail Chicken Meat in Ludhiana, India by Employing conventional Isolation Techniques and Molecular Polymerase Chain Reaction (PCR) Assay.Int.J.Curr.Microbiol.App.Sci. 9(7): 1510-1517. doi: https://doi.org/10.20546/ijcmas.2020.907.174