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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 1486-1489
DOI: https://doi.org/10.20546/ijcmas.2020.907.170


Introgression of Traits from Wild Diploids into Cultivated Tetraploids: A Pragmatic Analysis Using Genetic and Cytogenetic Tool
L. Mahalingam1*, N. Premalatha1, K. Senguttuvan1, B. Rakavi2 and M. Kumar3
1Department of Cotton, Centre for Plant Breeding and Genetics, Tamil Nadu Agricultural University, Coimbatore- 641 003, Tamil Nadu, India
2Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India
3Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
*Corresponding author
Abstract:

Interspecific triploid hybrid was developed between tetraploid cultivated species Gossypium hirsutum cv. MCU 5 and CO 14 and diploid wild species Gossypium anomalum. The F1 hybridity was confirmed by morphological and cytologicalstud. The ploidy level of interspecific F1 hybrid was triploid and male sterile. Maternal parents MCU 5 and CO 14 had erect growth habit, green stem, palmate leaves, thick and prominent leaf veins, cream petals and embedded stigma. MCU5 was with dense yellow anthers while CO 14 dense creamy anthers, whereas male parent Gossypium anomalum has spreading growth habit, dull violet petals, pale brownish green stem, creamy white, thin leaf veins, embedded stigma and strongly hairyness plant body. The growth habit, leaf shape and petal colour and petal size of interspecific F1 hybrids were similar to the paternal parent. Plant stem colour, hairiness, leaf pubescence and anther colour of Gossypium anomalum were observed to be dominant as hybrid fully resembled Gossypium anomalum for these characters. Petal spot was observed in Gossypium anomalum and in F1 hybrids while petal spot was not observed in MCU 5 and CO 14. Variable expression of petal spot, anther colour and filament colour was observed in the F1 hybrids. The mitotic study revealed that the maternal parent MCU 5 and CO 14 with 52 chromosomes, Gossypium anomalum had 26 chromosomes and the interspecific F1 was with 39 chromosomes. Significant differences were observed between pollen size, pollen fertility of parents and their hybrids. The F1 interspecific hybrids having more than 97 percent of sterile pollen grains. This F1 may be utilized as a pre breeding genetic resource for transfer of cotton jassid resistance to American cotton in breeding programme.


Keywords: Wild diploids, Cultivated tetraploids, Cytogenetic tool
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How to cite this article:

Mahalingam, L., N. Premalatha, K. Senguttuvan, B. Rakavi and Kumar, M. 2020. Introgression of Traits from Wild Diploids into Cultivated Tetraploids: A Pragmatic Analysis Using Genetic and Cytogenetic Tool.Int.J.Curr.Microbiol.App.Sci. 9(7): 1486-1489. doi: https://doi.org/10.20546/ijcmas.2020.907.170