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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2076-2080
DOI: https://doi.org/10.20546/ijcmas.2017.603.237


PCR Detection of Citrus Yellow Mosaic Virus (CYMV) and Citrus Greening Bacterium in Different Tissue of Infected Citrus Plant
K.N. Gupta1* and V.K. Baranwal2
1PC Unit AICRP Sesame and Niger, Jawaharlal Nehru Krishi Vishwa Vidyalaya,Jabalpur- 482 004, Madhya Pradesh, India
2Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute New Delhi –110012
*Corresponding author
Abstract:

Citrus Yellow Mosaic Virus (CYMV) disease and citrus greening bacterium are two important diseases in citrus. Citrus yellow mosaic virus (CYMV) disease is caused by bacilliform DNA virus while citrus greening disease caused by a fastidious bacterium (Candidatus liberibacter asiaticus) (Cla). Both the pathogens are infected citrus plants may detected by PCR using leaf tissues midrib tissue incase of greening. Both the disease are graft transmissible, and their relative distribution in tissues other than leaves are not known, we have demonstrated that CYMV and Cla can be detected by PCR in bark, and bud in addition to leaf tissue of infected citrus plants. These pathogens were absent in root tissue. It barks tissue can be a better tissue for DNA template preparation for PCR detection of CYMV. However, greening was amplified better in from leaf mid rib in comparison to bark tissue.               
 


Keywords: Citrus yellow mosaic virus, Greening bacterium, Molecular detection.
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How to cite this article:

Gupta, K.N. and Baranwal, V.K. 2017. PCR Detection of Citrus Yellow Mosaic Virus (CYMV) and Citrus Greening Bacterium in Different Tissue of Infected Citrus Plant.Int.J.Curr.Microbiol.App.Sci. 6(3): 2076-2080. doi: https://doi.org/10.20546/ijcmas.2017.603.237