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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 954-958
DOI: https://doi.org/10.20546/ijcmas.2020.907.111


Effect of 0.1% HgCl2 on Surface Sterilization of Som (Persea bombycina King) Explant during Tissue Culture - A Major Host Plant of Muga Silkworm
Junmoni Boruah*
Assam Agricultural University, Jorhat-13, Assam, India
*Corresponding author
Abstract:

In the current study we analysed the effect of 0.1% mercuric chloride (HgCl2) on surface sterilization of the explants at different durations of time (1-5mins). It was observed that, longer the duration of treatment, lower was the bacterial contamination (8% contamination at 5 min duration of HgCl2 treatment) but the survival percentage of the culture decreased as exposure time increased (35% at 5 min). 3 min of exposure gave the best result for both bacterial contamination (18%) and survival percentage (68%) among other time durations. Fungal contamination doesn’t showed any significant difference at different time intervals.


Keywords: Mercuric chloride, sterilization, duration, contamination
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How to cite this article:

Junmoni Boruah. 2020. Effect of 0.1% HgCl2 on Surface Sterilization of Som (Persea bombycina King) Explant during Tissue Culture - A Major Host Plant of Muga Silkworm.Int.J.Curr.Microbiol.App.Sci. 9(7): 954-958. doi: https://doi.org/10.20546/ijcmas.2020.907.111