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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 814-821
DOI: https://doi.org/10.20546/ijcmas.2020.907.094


Expression and Evaluation of Serological Reactivity of Recombinant TP53 Canine Mammary Tumour Associated Antigen
Shumaila Khan1,2, Sonal Saxena1, Poonam Singh2*, Nupur Dutta1, Sameer Srivastava1, Purnima Gogoi1 and Pallavi Shah1
1Department of Veterinary Biotechnology, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly (UP) – 243122, India
2Department of Molecular and Cellular Engineering, Sam Higginbottom University of Agriculture, Technology, and Sciences (SHUATS), Prayagraj (UP) – 211007, India
*Corresponding author
Abstract:

Canine Mammary Tumour (CMT) is the most common malignancy of unspayed female dogs and is of potential importance as a model for human breast cancer. Mortality rate in female dogs is three times than that of human females with breast cancer due to lack of detection at an early stage. Therefore it is important to identify candidate biomarkers for canine mammary tumour with potential for disease diagnosis at early stages. Tumour antigen P53 (TP53) is well established marker for human breast cancer studies, however few studies have focussed on its characterization in CMTs. Therefore in this study immunodominant region in TP53 gene was amplified and cloned in prokaryotic expression vector. Further the conditions were optimized for the expression of the recombinant protein. The recombinant protein was purified using Ni-NTA affinity chromatography. The hyperimmune sera were raised in rabbits against the recombinant protein. In the hyperimmune sera, antibodies specific to TP53 were detected by Indirect ELISA, confirming the serological reactivity of the immunodominant region. The antibodies raised against canine TP53 will be useful in developing assays for detecting TP53 antigen in serum of dogs with cancers. Further the purified antibodies can also be utilized for detection of TP53 antigen in CMT tissues.


Keywords: TP53, ELISA, PCR, cloning, CMT, Expression
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How to cite this article:

Shumaila Khan, Sonal Saxena, Poonam Singh, Nupur Dutta, Sameer Srivastava, Purnima Gogoi and Pallavi Shah. 2020. Expression and Evaluation of Serological Reactivity of Recombinant TP53 Canine Mammary Tumour Associated Antigen.Int.J.Curr.Microbiol.App.Sci. 9(7): 814-821. doi: https://doi.org/10.20546/ijcmas.2020.907.094