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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 621-627
DOI: https://doi.org/10.20546/ijcmas.2020.907.070


Characterization of Microbial Inoculants and its Different Formulation for Mass Production
Geeta Kumari1, Deepak Kumar1, Devendra Singh1,2* and Navnit Kumar3
1Department of Microbiology, College of Basic Sciences and Humanities,
Dr. Rajendra Prasad Central Agricultural University, Pusa, Samasitpur, Bihar, 848125, India
2ICAR-Directorate of Medicinal and Aromatic Plants Research, Boriavi, Anand, Gujarat-387310, India
3Department of Agronomy, Sugarcane Research Institute, Dr. Rajendra Prasad Central Agricultural University, Pusa, Samasitpur, Bihar, 848125, India
*Corresponding author
Abstract:

Biofertilizers are alternative source of nutrient inputs in sustainable agriculture. Plant growth promoting microorganisms such as Rhizobium spp. and Bacillus spp. can be used as biofertilizers. In our present study, different Rhizobium and Bacillus isolates were screened for the selection of suitable medium for its maximum growth at different incubation period. The Growth pattern of Rhizobium isolates were evaluated in two different media viz. Nutrient broth and YEM broth while of Bacillus spp. on four different media viz. LGD broth, Nutrient broth, NYSM broth and BMS broth. Our results revealed that the YEM broth supported better growth of Rhizobium isolates than Nutrient broth. However, the maximum growth of Bacillusspp. was observed in BMS broth while least in LGD broth at O.D 620nm after 72 hrs.But there was the problem to take optical density because of formation of black pellicle in BMS media byBacillus spp. Therefore, we conclude that NYSM media is best media for the growth of Bacillus spp. Rhizobium and Bacillus spp. were evaluated for IAA production. Bacillus spp. was found more efficient than Rhizobium in terms of IAA production. For different carrier materials like Charcoal, Fly-ash and additives such as Glycerol and Trehalose were used over a period of 15 days of storage to assess the survivability of different Rhizobium and Bacillus isolates. Based on the finding of the study, the charcoal can be used as an alternate carrier material to prepare the microbial inoculants. However, the liquid inoculants were found to be better in supporting the population of inoculants than the carrier based inoculants.


Keywords: Carrier materials, Additives, Microbial inoculants, Growth pattern, IAA production
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How to cite this article:

Geeta Kumari, Deepak Kumar, Devendra Singh and Navnit Kumar. 2020. Characterization of Microbial Inoculants and its Different Formulation For Mass Production.Int.J.Curr.Microbiol.App.Sci. 9(7): 621-627. doi: https://doi.org/10.20546/ijcmas.2020.907.070