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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 507-519
DOI: https://doi.org/10.20546/ijcmas.2020.907.056


Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.) from Agricultural Soils of Daloa Localities in Côte D’ivoire
Guei Nassé Kaéda Raissa1, Konate Ibrahim1¬, Taha Kaoutar2, Bamba Issouf1, Beugre Grah Avit Maxwell1, Akaffou Doffou Sélastique1 and Filali-Maltouf Abdelkarim2
1UFR Agro-forestry and Environment, Department of Biochemistry and Microbiology, Laboratory of Agrovalorization, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire
2Laboratory of Microbiology and Molecular Biology, Faculty of Sciences,
University Mohammed V in Rabat, Morocco
*Corresponding author
Abstract:

Bambara groundnut (Vigna subterranea L. Verdc.) is an indigenous, drought-tolerant, underutilized African food legume, with the ability to fix atmospheric N2 in symbiosis with soil rhizobial bacteria. Our work aimed to analyze the genetic diversity of 15 bacteria isolated from root nodules of Bambara groundnut coded RVC in Côte d’Ivoire and to evaluate their symbiotic characteristic in order to select the most efficient strains as biofertilizers. Molecular analysis of these isolates using 16S rRNA gene sequences revealed distinct evolutionary lineages related to the genus Rhizobium (80%), Bradyrhizobium (13.3%) and Ensifer (6.7%). Specifically, these isolates were very closed to B. yuanmingense, Rhizobium sp. A2Ec4, Rhizobium sp. HIN2, R. alamii CK-8, R. sullae SCAU26, Rhizobium sp. UFLA 02-232, Rhizobium sp.UFPI-38 and Ensifer sp. PZS-S05. The nodulation test showed significant differences (P<0.05) in nodule number (18 and 87/plant) and dry matter (21.67 and 289.33 mg/plant). Besides, the isolates RVC25, RVC50, and RVC13 exhibited the highest infectious capacity with a means of symbiotic effectiveness ranged from 42 to 83%. The isolate RVC25 was scored as highly effective (83%). Thus the strain RVC25 characterized as R. alamii was identified as having interesting symbiotic features to improve the Bambara groundnut production. Symbiotic parameters had a significant contribution to growth, health and yield of the host plant.


Keywords: Bambara groundnut, Rhizobia, symbiotic efficiency, 16S-rRNA, phylogeny
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How to cite this article:

Guei Nassé Kaéda Raissa, Konate Ibrahim, Taha Kaoutar, Bamba Issouf, Beugre Grah Avit Maxwell, Akaffou Doffou Sélastique and Filali-Maltouf Abdelkarim. 2020. Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.) from Agricultural Soils of Daloa Localities in Côte D’ivoire.Int.J.Curr.Microbiol.App.Sci. 9(7): 507-519. doi: https://doi.org/10.20546/ijcmas.2020.907.056