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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 346-354
DOI: https://doi.org/10.20546/ijcmas.2020.907.037


In-Vitro Efficacy of Forms and Methods of Adhatoda vasica against Flacherie causing Bacteria of Silkworm, Bombyx mori L.
K. V. Anitharani*, R. N. Bhaskar and H. G. Anusha
Department of Sericulture, UAS GKVK, Bengaluru-560065 (Karnataka), India
*Corresponding author
Abstract:

The flacherie diseased silkworms may contain multiple species of bacteria viz., Streptococcus faecalis, Staphylococcus aureus, Serratia marcescens and Bacillus sp. The use of botanical formulations possessing antibacterial activity are preferred in silkworm rearing over the chemical agents which found to be effective on checking bacterial infection and enhance the growth of silkworm. Different forms and methods of application of Adhatoda vasica was found effective in the management of bacterial flacherie of silkworm. In the present study, three bacterial species (Bacillus sp., Staphylococcus sp. and Streptococcus sp.) were isolated from haemolymph of silkworm and used under in-vitro condition. The study revealed that, the aqueous, acetone and alcoholic extract of A.vasica at different concentrations (2, 4 and 6 %) were highly effective against three bacterial species. However, the maximum zone of inhibition was observed in alcoholic extract (8.00, 9.12 and 8.99, 9.62 mm)(7.42, 7.88 and 7.84, 8.63 mm) (8.25, 8.87 and 8.84, 9.21 mm) and minimum in aqueous extract(7.66, 8.46 and 8.25, 8.94 mm) (6.96 and 7.29., 7.35 and 7.79 mm) (6.46, 6.92 and 8.09, 8.46 mm) on 24 and 48 hours of incubationagainst10-5 and 10-7 spore dilution of Bacillus, Staphylococcus and Streptococcus species, respectively.


Keywords: Adhatodavasica, bacterial flacherie, inhibition zone.
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How to cite this article:

Anitharani. K. V., R. N. Bhaskar and Anusha. H. G. 2020. In-Vitro Efficacy of Forms and Methods of Adhatoda vasica against Flacherie causing Bacteria of Silkworm, Bombyx mori L.Int.J.Curr.Microbiol.App.Sci. 9(7): 346-354. doi: https://doi.org/10.20546/ijcmas.2020.907.037