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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 189-196
DOI: https://doi.org/10.20546/ijcmas.2020.907.022


Comparative Evaluation of Rapid Crossing Techniques for Wheat x Maize System of Doubled Haploid Production
Gagandeep Singh, Puja Srivastava and G. S. Mavi*
Wheat section, Department of Plant breeding & Genetics, PAU, Ludhiana, India
*Corresponding author
Abstract:

Five different emasculation methods viz. standard technique for wheat x maize crosses, early pollinations on non-emasculated spikes, clipped glume facilitated emasculation, plastic bag emasculation and hot water emasculation were compared. Clipped glume facilitated emasculation resulted in lowest caryopses formation frequency. Early pollination on non-emasculated spikes gave reasonably high embryo formation frequency while omitting tedious emasculation steps. An innovative method based on non-dehiscence and functional sterility of anthers exposed to high humidity in plastic bag showed good promise though considerable proportion of selfed seed (32.99%) was observed. The method that proved best involved hot water (430 C for 4 min) treatment of detached tillers. It showed high embryo formation frequency (30.88 %) and low selfed seed set (3.20%). Some level of female sterility observed in this method is needed to be tackled through further refinement.


Keywords: Wheat, maize, Plastic bag, clipped glume
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How to cite this article:

Gagandeep Singh, Puja Srivastava and G. S. Mavi. 2020. Comparative Evaluation of Rapid Crossing Techniques for Wheat x Maize System of Doubled Haploid Production.Int.J.Curr.Microbiol.App.Sci. 9(7): 189-196. doi: https://doi.org/10.20546/ijcmas.2020.907.022