National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Five different emasculation methods viz. standard technique for wheat x maize crosses, early pollinations on non-emasculated spikes, clipped glume facilitated emasculation, plastic bag emasculation and hot water emasculation were compared. Clipped glume facilitated emasculation resulted in lowest caryopses formation frequency. Early pollination on non-emasculated spikes gave reasonably high embryo formation frequency while omitting tedious emasculation steps. An innovative method based on non-dehiscence and functional sterility of anthers exposed to high humidity in plastic bag showed good promise though considerable proportion of selfed seed (32.99%) was observed. The method that proved best involved hot water (430 C for 4 min) treatment of detached tillers. It showed high embryo formation frequency (30.88 %) and low selfed seed set (3.20%). Some level of female sterility observed in this method is needed to be tackled through further refinement.
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