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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(7): 80-89
DOI: https://doi.org/10.20546/ijcmas.2020.907.009


Detection and Characterization of Multi Drug-resistant Extended-spectrum and pAmpC Beta-lactamases Producing Escherichia coli from Chicken Meat in West Bengal, India
Kunal Batabyal1*, Abhiroop Banerjee1, Samir Dey1, Indranil Samanta1, Devi Prasad Isore1 and Abhishek Dharm Singh2
1Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences,
West Bengal University of Animal and Fishery Sciences, Kolkata – 37, West Bengal, India
2Department of Veterinary Public Health, F/VAS, West Bengal University of Animal and Fishery Sciences, Kolkata – 37, West Bengal, India
*Corresponding author
Abstract:

Antimicrobial resistance can be seen in almost all pathogenic bacteria present in food like chicken meat, leading to treatment failure in human patients and serious public health problems. The present study aimed to detect extended-spectrum beta-lactamases (ESBLs) and AmpC beta-lactamase (ACBL)-producing Escherichia coli from chicken meat, from different parts of West Bengal. A total of 113 raw chicken meat samples were collected during slaughter from different local markets followed by isolation and identification by standard conventional and molecular methods. About79% samples were positive for E. coli and among 89 isolates 17(19.1%) were positive to ESBL property and presence of the blaCTX-M gene, whereas 78 strains (87.6%) were found to possess blaAmpC gene. Antibiogram study of ESBL positive E. coli strains revealed resistance of these strains to ceftriaxone, ampicillin (both 100%), amoxicillin/clavulanic acid, ceftazidime, cefotaxime, tetracycline (all approx. 94%), azithromycin (70.6%) and norfloxacin (64.7%) in-vitro whereas imipenem (94%), amikacin (82%), gentamicin (58.8%) and ampicillin/sulbactam (71%) were quite effective against these MDR isolates. So, about 79% of chicken meat samples were found to be contaminated with E. coli, most of which were resistant to commonly used antibiotics which may lead to animal and human health hazards.


Keywords: Antibiogram, blaAmpC, blaCTX-M, Chicken meat, E. coli, ESBL
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How to cite this article:

Kunal Batabyal, Abhiroop Banerjee, Samir Dey, Indranil Samanta, Devi Prasad Isore and Abhishek Dharm Singh. 2020. Detection and Characterization of Multi Drug-resistant Extended-spectrum and pAmpC Beta-lactamases Producing Escherichia coli from Chicken Meat in West Bengal, India.Int.J.Curr.Microbiol.App.Sci. 9(7): 80-89. doi: https://doi.org/10.20546/ijcmas.2020.907.009