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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3764-3768
DOI: https://doi.org/10.20546/ijcmas.2020.906.445


Optimization of Plaque Forming Conditions for an Aeromonas hydrophila Lytic Bacteriophage
Sumeet Rai, Anuj Tyagi1*, B. T. Naveen Kumar1 and Niraj Kumar Singh2
1 Department of Aquatic Environment, College of Fisheries,
2College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India
*Corresponding author
Abstract:

Recent emergence of antibiotic resistant bacteria has led to renewed interest in the phage research for control of aquatic bacterial pathogens. Plaque assay is an important part of phage research. For some phages, the plaques could be very much turbid and small in size resulting in difficulties during phage isolation and enumeration. Present study investigated the effect of soft agar as well as Ca++ and Mg++ ion concentrations on plaque forming ability of a Aeromonas hydrophila lytic phage. At 0.2% soft agar, with 10 mM of CaCl2 and MgSO4, mean ± SD plaque size of 5.55 ± 0.52 mm and counts of (58 ± 4) were significantly higher the other concentrations. However, increase of agar concentration by value of just 0.1% reduced the plaque diameters to less than one millimeter. No plaques could be observed at soft agar concentration of ≥4%. High CaCl2 and MgSO4 concentrations also affected plaque morphology, as plaques were found to be very much uniform and clear up to 25 mM, but turned to turbid and irregular size at 100 mM of CaCl2 and MgSO4. This study provides important insights to optimize plaque assay for phages forming turbid and micro sizes plaques leading to their efficient utilization in phage therapy in aquaculture.


Keywords: Aeromonas hydrophila, Phage, Plaque assay, Soft agar, Ionic concentration
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How to cite this article:

Sumeet Rai, Anuj Tyagi, B. T. Naveen Kumar and Niraj Kumar Singh. 2020. Optimization of Plaque Forming Conditions for an Aeromonas hydrophila Lytic Bacteriophage.Int.J.Curr.Microbiol.App.Sci. 9(6): 3764-3768. doi: https://doi.org/10.20546/ijcmas.2020.906.445