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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3630-3638
DOI: https://doi.org/10.20546/ijcmas.2020.906.427


Isolation and Identification of Candida Species from Various Clinical Samples and their Biofilm Production Status
S. Prateeksha1, D.C. Shwetha2* and D. Venkatesha2
1Adichunchanagiri Institute of Medical Sciences, AIMS, BG nagara, Mandya -571448, India
2Department of Microbiology, AIMS, B G Nagara, Mandya- 571448, India
*Corresponding author
Abstract:

Candida species are the most common fungal species causing mucosal and systemic infections. Candida albicans has been the most common causative agent affecting mostly the immunocompromised. However, an increase in the prevalence of Non Albicans Candida (NAC) species is noted in the recent two decades. In this study Candida species from various clinical samples were isolated and their biofilm production status was evaluated. All the clinical samples were inoculated on to Blood agar and MacConkey agar. The suspected colonies of Candida were further confirmed by Gram stain. Germ tube test was done for the differentiation of C. albicans and C. dublinensis from the other candida species. It was further inoculated onto HiCrome Candida Differential agar for the speciation. The Biofilm production was done using the Congo-red agar. Out of a total 36 candida species that were isolated during the study, 18 (50%) were found to be Candida albicans and remaining 18 were found to be NAC (22.2% were C. tropicalis, 19.4% were C. glabrata, 8.4% were C. krusei). Among the 36 candida species, 12 (33%) were biofilm producers while the remaining 24 (64%) were non-biofilm producers. This study showed that there is an increase in the pathogenicity and prevalence of the NAC species.


Keywords: Candida species, Clinical samples, Biofilm production status
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How to cite this article:

Prateeksha, S., D.C. Shwetha and Venkatesha, D. 2020. Isolation and Identification of Candida Species from Various Clinical Samples and their Biofilm Production Status.Int.J.Curr.Microbiol.App.Sci. 9(6): 3630-3638. doi: https://doi.org/10.20546/ijcmas.2020.906.427