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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3471-3482
DOI: https://doi.org/10.20546/ijcmas.2020.906.409


Virulent Gene Characterization of Methicillin Sensitive Staphylococcus aureus (MSSA) and Methicillin Resistant Staphylococcus aureus (MRSA) from Bovine Mastitis
Hamid Shah1*, Mohd Altaf Bhat1, Burhan Nabi2, Anil Taku1 and Gulzar Ahmed Badroo1
1Divison of Veterinary Microbiology and Immunology, F.V.Sc & A.H., SKUAST-J, India
2Division of Veterinary Medicine, F.V.Sc & A.H, SKUAST-J, India
*Corresponding author
Abstract:

The objective of the study was to characterize the virulence gene profile of MSSA and MRSA isolated from bovine mastitis. A total of 160 mastitic milk samples were collected from different regions of Jammu. Following different molecular techniques, a total of 36(37.5%) isolates were confirmed as S. aureus. These 36 S. aureus isolates were inoculated on ORSA to determine the phenotypic MRSA isolates. Only 20(55.5%) isolates were confirmed to be MRSA phenotypically. All 36 isolates were subjected to mecA gene amplification by PCR. Only 6 isolates were mecA gene positive hence MRSA. Both MRSA and MSSA isolates were screened for virulence genes viz. coagulase (coa), staphylococcal protein A(spa), clumping factor (clfA), haemolysin A(hla) and enterotoxins (eta &etb). The coa gene was present in 35(97.2%), spa in 35(97.2%), clfA in 35(97.2%), hla in 22(61.1%) and eta &etb in none of the isolates.


Keywords: Amplification, Gene, Isolates, Molecular, PCR, Virulence
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How to cite this article:

Hamid Shah, Mohd Altaf Bhat, Burhan Nabi, Anil Taku and Gulzar Ahmed Badroo. 2020. Virulent Gene Characterization of Methicillin Sensitive Staphylococcus aureus (MSSA) and Methicillin Resistant Staphylococcus aureus (MRSA) from Bovine Mastitis.Int.J.Curr.Microbiol.App.Sci. 9(6): 3471-3482. doi: https://doi.org/10.20546/ijcmas.2020.906.409