National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The objective of the study was to characterize the virulence gene profile of MSSA and MRSA isolated from bovine mastitis. A total of 160 mastitic milk samples were collected from different regions of Jammu. Following different molecular techniques, a total of 36(37.5%) isolates were confirmed as S. aureus. These 36 S. aureus isolates were inoculated on ORSA to determine the phenotypic MRSA isolates. Only 20(55.5%) isolates were confirmed to be MRSA phenotypically. All 36 isolates were subjected to mecA gene amplification by PCR. Only 6 isolates were mecA gene positive hence MRSA. Both MRSA and MSSA isolates were screened for virulence genes viz. coagulase (coa), staphylococcal protein A(spa), clumping factor (clfA), haemolysin A(hla) and enterotoxins (eta &etb). The coa gene was present in 35(97.2%), spa in 35(97.2%), clfA in 35(97.2%), hla in 22(61.1%) and eta &etb in none of the isolates.
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