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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 3158-3164
DOI: https://doi.org/10.20546/ijcmas.2020.906.378


Molecular Characterization of Maize (Zea mays L.) Inbred Lines using Simple Sequence Repeats (SSR) Marker
Urusha Zareen1, Birender Singh1*, Bishun Deo Prasad2, S. S. Mandal1, R. B. P. Nirala1 and Girish Nandan1
1Department of Plant Breeding and Genetics
2Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur – 813210, India
*Corresponding author
Abstract:

Genomic DNA was isolated by CTAB method and then subjected to PCR amplification by using 22 SSR primers. Among 22 primers, 17 primers were amplified. The PIC values were ranged from 0.46 (Phi046) which is the minimum value to 0.81(Phi059) which is the highest value. The frequencies of alleles were ranged from 0.01 to 0.07. By the use of Unweighted Paired Group Method (UGPMA) for cluster analysis and formed two cluster. DHM117 showed 65per cent similarity with CLQRCY44 and CLO2450 showed 3per cent similarity with BML-6 which is the minimum value. In this study, PIC value indicated a good efficiency of marker for studying the polymorphism level available in studied inbred line and it also indicated their suitability for further breeding programs.


Keywords: Maize, Zea mays, SSR marker, PIC, UGPMA
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How to cite this article:

Urusha Zareen, Birender Singh, Bishun Deo Prasad, S. S. Mandal, R. B. P. Nirala and Girish Nandan. 2020. Molecular Characterization of Maize (Zea mays L.) Inbred Lines using Simple Sequence Repeats (SSR) Marker.Int.J.Curr.Microbiol.App.Sci. 9(6): 3158-3164. doi: https://doi.org/10.20546/ijcmas.2020.906.378