National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Genomic DNA was isolated by CTAB method and then subjected to PCR amplification by using 22 SSR primers. Among 22 primers, 17 primers were amplified. The PIC values were ranged from 0.46 (Phi046) which is the minimum value to 0.81(Phi059) which is the highest value. The frequencies of alleles were ranged from 0.01 to 0.07. By the use of Unweighted Paired Group Method (UGPMA) for cluster analysis and formed two cluster. DHM117 showed 65per cent similarity with CLQRCY44 and CLO2450 showed 3per cent similarity with BML-6 which is the minimum value. In this study, PIC value indicated a good efficiency of marker for studying the polymorphism level available in studied inbred line and it also indicated their suitability for further breeding programs.
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