National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Expression of efflux pumps and extrusion of antibiotics from bacterial cell through them is one of important innate causes of antimicrobial resistance. The present study aimed to design synthetic peptides as efflux pump inhibitors. Two synthetic peptides SG-15 and GG-15 were designed on the basis of protein sequence and structure of AcrAB-TolC a commonly expressed efflux pump of Escherichia coli (E. coli). SG-15 was designed as inner membrane protein (AcrB) blocker and GG-15 was designed as outer membrane protein (TolC) blocker. The designed inhibitors were custom synthesized. The synergistic effect of these peptides with three antibiotics namely; Ampicillin, Gentamicin and Cephalothin was evaluated through minimum inhibitory concentration of antibiotics alone and with peptides on 10 E. coli isolates obtained from mastitic milk samples of cattle. On testing of synergistic effect SG-15 has given better synergistic effect with all the three antibiotics than GG-15 against E. coli but, the extent of effect varied between different antibiotics. Combination of Ampicillin and GG-15 did not show synergistic effect. Interestingly, there was significant synergistic effect on combination of both the peptides with these antibiotics. The designed peptides have shown potent efflux inhibition activity, possibly by blocking the channel present in commonly expressed efflux pump, AcrAB-TolC of E. coli. These peptides have great potential for their synergistic use with antibiotics to reduce efflux pumps mediated antibiotic resistance.
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