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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2867-2876
DOI: https://doi.org/10.20546/ijcmas.2020.906.347


Synergistic Effect of Some Synthetic Peptides and Antibiotics on Efflux Pump of Escherichia coli
Basant1*, Rekha Panwar1, Parma Ram Gorachiya2, Brij Nandan Shringi1, Sunil Maherchandaniand Amit Kumar Pandey3
1Department of Veterinary Microbiology and Biotechnology,
2Department of Livestock Products Technology,
3Department of Veterinary Biochemistry, University of Veterinary and Animal Sciences, Bikaner (Rajasthan), India
*Corresponding author
Abstract:

Expression of efflux pumps and extrusion of antibiotics from bacterial cell through them is one of important innate causes of antimicrobial resistance. The present study aimed to design synthetic peptides as efflux pump inhibitors. Two synthetic peptides SG-15 and GG-15 were designed on the basis of protein sequence and structure of AcrAB-TolC a commonly expressed efflux pump of Escherichia coli (E. coli). SG-15 was designed as inner membrane protein (AcrB) blocker and GG-15 was designed as outer membrane protein (TolC) blocker. The designed inhibitors were custom synthesized. The synergistic effect of these peptides with three antibiotics namely; Ampicillin, Gentamicin and Cephalothin was evaluated through minimum inhibitory concentration of antibiotics alone and with peptides on 10 E. coli isolates obtained from mastitic milk samples of cattle. On testing of synergistic effect SG-15 has given better synergistic effect with all the three antibiotics than GG-15 against E. coli but, the extent of effect varied between different antibiotics. Combination of Ampicillin and GG-15 did not show synergistic effect. Interestingly, there was significant synergistic effect on combination of both the peptides with these antibiotics. The designed peptides have shown potent efflux inhibition activity, possibly by blocking the channel present in commonly expressed efflux pump, AcrAB-TolC of E. coli. These peptides have great potential for their synergistic use with antibiotics to reduce efflux pumps mediated antibiotic resistance.


Keywords: Escherichia coli, Efflux pump, SG-15 and GG-15
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How to cite this article:

Basant, Rekha Panwar, Parma Ram Gorachiya, Brij Nandan Shringi, Sunil Maherchandani and Amit Kumar Pandey. 2020. Synergistic Effect of Some Synthetic Peptides and Antibiotics on Efflux Pump of Escherichia coli.Int.J.Curr.Microbiol.App.Sci. 9(6): 2867-2876. doi: https://doi.org/10.20546/ijcmas.2020.906.347