National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
β-galactosidase enzyme is commercially important and is generally used in dairy industries for the improvement of lactose intolerance. In this study, different strains of isolated lactic acid bacteria were evaluated for their β-galactosidase productivity, but Lactobacillus casei MB2resulted with the highest production. Therefore, optimal conditions were determined in submerged fermentation processes by using the “one factor at a time” approach. The optimal cultivation conditions predicated from this method were achieved by using MRS medium on 2ndday, pH 7.0, temperature 30ºC with inoculum size of 4%, lactose as carbon source, peptone as nitrogen source with 3% substrate (lactose) concentration, Mg 2+ as divalent ions and Tween 20 as surfactant added with an approximate yield of 139.65 IU/ml escalating the level of β-galactosidase production.
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