National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The present study was to determine the expression of proliferating cell nuclear antigen protein (PCNA) in the sheep and goat ovaries. The localization of PCNA was demonstrated in 5µm thick formalin fixed, paraffin embedded tissue sections of sheep and goat ovarian tissue using primary mouse monoclonal anti-PCNA antibody. Staining for PCNA was not observed in pregranulosa cells but observed in the oocytes of primordial follicles. In primary to secondary follicles, positive staining in oocytes and in some granulosa cells was detected. The preantral follicles and actively growing small to large antral follicles showed strong PCNA labelling in the layers of granulosa, and theca cells. PCNA staining was expressed in nuclei of oocytes in preantral and small antral follicles. In atretic follicles, the level of PCNA protein expression was negatively correlated on the stage of atresia. The follicles demonstrating advanced atresia showed mild or no PCNA labelled granulosa and theca cells. The results of the study demonstrate that follicular growth and development in ovaries may be effectively monitored by determining the rate of granulosa cell proliferation of PCNA.
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