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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2594-2608
DOI: https://doi.org/10.20546/ijcmas.2020.906.316


Identification and Optimization of Fermentation Medium for Production of Antibacterial Compounds from Endophytic Streptomyces sp. GBTPR-167
Pushpendra Sharma, Shweta Ranghar and Mamta Baunthiyal*
Department of Biotechnology, Govind Ballabh Pant Institute of Engineering & Technology, Ghurdauri, Pauri (Garhwal), Uttarakhand, India-246194
*Corresponding author
Abstract:

Nutritional and environmental conditions are important parameters that influence growth and secondary metabolite production by actinobacteria. Endophytic actinobacteria (n=283) isolated from different tissues (root, needle and stem) of Pinus roxburghii of Pauri (Garhwal) region, India, were screened for their antibacterial potential. A potent endophytic actinobacterial strain, GBTPR-167, isolated from root tissue of P. roxburghii was identified on the basis of morphological, chemotaxonomic and 16S rDNA analysis as Streptomyces sp. The strain was found to produce bioactive compound with high antibacterial activity in FM-2 medium. OFAT based optimization of factors effect the production of bioactive compound and maximum production was observed in medium containing glucose as carbon source and casein as nitrogen source at pH 7.0, 180 rpm and 30ºC on 7th day of incubation. Further, Streptomyces sp. displayed salt tolerance up to 6% NaCl concentration however; maximum antibacterial activity was obtained at 2% NaCl concentration. The study holds importance as enhanced production of antibacterial compound can be achieved with these improved parameters. Also, optimization strategies may be undertaken by using statistical tools like design expert.


Keywords: Endophytic actinobacteria; Streptomyces sp.; Optimization; Fermentation medium;
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How to cite this article:

Pushpendra Sharma, Shweta Ranghar and Mamta Baunthiyal. 2020. Identification and Optimization of Fermentation Medium for Production of Antibacterial Compounds from Endophytic Streptomyces sp. GBTPR-167.Int.J.Curr.Microbiol.App.Sci. 9(6): 2594-2608. doi: https://doi.org/10.20546/ijcmas.2020.906.316