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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2250-2259
DOI: https://doi.org/10.20546/ijcmas.2020.906.275


Pyomelanin Production From a Marine Isolate of Acinetobacter Spp.
Jean Loi1, Tan Shi Yi1, Attapol Pinsa1, Sakinah Mulyana1, Lloyd George Singaretnam2, Meliana Riwantoand Jason Chang1*
1School of Applied Science, Temasek Polytechnic, 21 Tampines Avenue 1, Singapore 529757 2Present address: School of Applied Science, Republic Polytechnic, 9 Woodlands Avenue 9, Singapore 738964
*Corresponding author
Abstract:

There is growing interest in the use of natural pigments for a wide range of applications. A search for pigment-producing bacteria led to the isolation of a marine bacterium that produced a diffusible brown pigment. The bacterium was identified as Acinetobacter spp.from 16S rRNA gene sequencing. The pigment displayed free-radical scavenging activity and was characterized as a melanin through solubility tests, UV-Vis spectrophotometry and Fourier transform infrared (FTIR) spectroscopy. Melanin production was blocked by sulcotrione, an inhibitor of 4-hydroxyphenylpyruvate dioxygenase (HPPD), the enzyme responsible for producing homogentisic acid (HGA), which is the monomeric precursor of pyomelanin. Inactivation of melanin synthesis by HPPD inhibition confirmed that the pigment was pyomelanin. Acinetobacter spp. required at least 12 days to produce approximately 1g/L of pyomelanin when it was grown in basal medium supplemented with L-tyrosine. The rate of pyomelanin production was not influenced by nutrient levels in growth media. Supplementation with different carbon sources and the use of nutrient-rich media had no effect in accelerating pigment production. However, pH had a major effect on pyomelanin formation by significantly reducing the time taken to produce the pigment. When the pH of growth medium (pH 5.5) was adjusted to pH 8, bacteria were able to produce pyomelanin in 7 days instead of 12-14 days.


Keywords: Bacterial pigments, Acinetobacter spp., Pyomelanin production, pH
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How to cite this article:

Jean Loi, Tan Shi Yi, Attapol Pinsa, Sakinah Mulyana, Lloyd George Singaretnam, Meliana Riwanto and Jason Chang. 2020. Pyomelanin Production From a Marine Isolate of Acinetobacter Spp..Int.J.Curr.Microbiol.App.Sci. 9(6): 2250-2259. doi: https://doi.org/10.20546/ijcmas.2020.906.275