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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2228-2233
DOI: https://doi.org/10.20546/ijcmas.2020.906.272


Molecular Detection of Bacillus anthracis in Beef Sample Recovered from an Anthrax‒Endemic Village of India
N. Sahoo1*, D. Hemadri2, S. Sahoo2, B. Behera1, A. Pahari1, M. Dash1, S. Satpathy3 and M. Dolai3
1Centre for Wildlife Health, Odisha University of Agriculture and Technology, India
2Indian Council of Agricultural Research‒National
Institute of Veterinary Epidemiology and Disease information, India
3Department of Animal Husbandry and Veterinary Services, Koraput, Odisha, India
*Corresponding author
Abstract:

Anthrax is a bacterial zoonoses caused by Bacillus anthracis that primarily affects herbivores, especially cattle. Human incidence is preceded by outbreaks in animals where infection is acquired through contact or ingestion of contaminated meat, or animal products. Present study deals with TaqMan real time PCR detection of B. anthracis in uncooked beef sample collected from an anthrax‒endemic village in Boipariguda block of Koraput district, Odisha, India following reports of cutaneous anthrax in humans. Meat samples of a 7 years old bullock with signs of pyrexia and bloat was traced from the area where the carcass was de-skinned and disbursed. Molecular test revealed presence of capsule (Cap) and protective antigen (PA) of B. anthracis. Characteristic sigmoid curves were observed for both the genes. Cap gene-TaqMan PCR of the test sample had a Ct value of 23.53 whereas positive control had a value of 17.81. Ct values were 17.22 and 24.03 for the positive control and the test sample, respectively. Positive results obtained for both the genes indicated the presence of a virulent strain in the area under study. Source of human anthrax with its mode of transmission demands strengthening of vaccination to livestock and awareness program in the area.


Keywords: Bacillus anthracis, bullock, meat, real time PCR
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How to cite this article:

Sahoo. N., D. Hemadri, S. Sahoo, B. Behera, A. Pahari, M. Dash, S. Satpathy and Dolai. M. 2020. Molecular Detection of Bacillus anthracis in Beef Sample Recovered from an Anthrax‒Endemic Village of India.Int.J.Curr.Microbiol.App.Sci. 9(6): 2228-2233. doi: https://doi.org/10.20546/ijcmas.2020.906.272