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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(6): 2195-2203
DOI: https://doi.org/10.20546/ijcmas.2020.906.268


Evaluation of Various Methods for Detecting Biofilm Formation among the Clinical Isolates of Staphylococcus species
Shruti Nair1 and S. Megha2*
1Adichunchanagiri Institute of Medical Sciences, B.G. Nagar, Mandya-571448, India 2Department of Microbiology, Adichunchanagiri Institute of Medical Sciences, B.G. Nagar, Mandya-571448, India
*Corresponding author
Abstract:

Biofilm producing strains of bacteria have become major cause for many chronic and persistent infections due to their increased antimicrobial resistance patterns. It is hence important to detect them as early as possible to administer appropriate treatment. The objective of this study is to compare the three conventionally used methods for biofilm detection, namely, Tissue culture plate, Tube adherence and Congo red agar method. 109 clinical isolates of Staphylococcus species were collected from various clinical samples and biofilm detection was checked for each by all of the above mentioned methods. Of these, 45 were identified as Staphylococcus aureus (SA) and 64 as Coagulase Negative Staphylococci (CONS). Positive biofilm producers by TCP method were 75(68.81%), 41(37.61%) by tube adherance method and 13(11.93%) by CRA method. Statistical analysis was done considering TCP method as gold standard and by using Chi square test. For tube adherence method sensitivity was 23.3%, specificity 52.9% positive predictive value 60.9%, negative predictive value 26.5% and p value was 0.171. For CRA method sensitivity was 14.6%, specificity 94.1% positive predictive value 84.6%, negative predictive value 33.3% and p value was 0.190. TCP method was concluded to be the most sensitive, accurate and reproducible screening method among the three studied for detection of biofilm formation among Staphylococcal species.


Keywords: Biofilm, tissue culture plate method, congo red agar, tube adherence, Staphylococcus
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How to cite this article:

Shruti Nair and Megha. S. 2020. Evaluation of Various Methods for Detecting Biofilm Formation among the Clinical Isolates of Staphylococcus Species.Int.J.Curr.Microbiol.App.Sci. 9(6): 2195-2203. doi: https://doi.org/10.20546/ijcmas.2020.906.268