National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Biofilm producing strains of bacteria have become major cause for many chronic and persistent infections due to their increased antimicrobial resistance patterns. It is hence important to detect them as early as possible to administer appropriate treatment. The objective of this study is to compare the three conventionally used methods for biofilm detection, namely, Tissue culture plate, Tube adherence and Congo red agar method. 109 clinical isolates of Staphylococcus species were collected from various clinical samples and biofilm detection was checked for each by all of the above mentioned methods. Of these, 45 were identified as Staphylococcus aureus (SA) and 64 as Coagulase Negative Staphylococci (CONS). Positive biofilm producers by TCP method were 75(68.81%), 41(37.61%) by tube adherance method and 13(11.93%) by CRA method. Statistical analysis was done considering TCP method as gold standard and by using Chi square test. For tube adherence method sensitivity was 23.3%, specificity 52.9% positive predictive value 60.9%, negative predictive value 26.5% and p value was 0.171. For CRA method sensitivity was 14.6%, specificity 94.1% positive predictive value 84.6%, negative predictive value 33.3% and p value was 0.190. TCP method was concluded to be the most sensitive, accurate and reproducible screening method among the three studied for detection of biofilm formation among Staphylococcal species.
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