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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1506-1518
DOI: https://doi.org/10.20546/ijcmas.2017.603.173


Flow Cytometric Detection of Natural Killer Cells and Natural Killer T Cells Changes in the Blood of Patients with Chronic Hepatitis C Virus Infection in an Egyptian University Hospital
Marwa Mostafa Shalaby1*, Kareman Ahmed Eshra1, Radwa Mahmoud Sharaby2 and Ibrahim Fathy Amer3
1Department of Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt
2 Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt
3 Department of Hepatology, Gastroentrology and Infectious Diseases, Faculty of Medicine, Kafr El-Sheikh University, Egypt
*Corresponding author
Abstract:

Hepatitis C virus (HCV) infects many people worldwide and it is a leading cause of cirrhosis and hepatocellular carcinoma. Natural Killer (NK) cells have an important role during HCV infection as a part of innate immune responses. Three subtypes of NK cells are recognized: CD56dim CD16+ve, CD56bright CD16+ve and CD56–veCD16+ve. Natural killers T (NKT) (CD3CD16CD56+ve) cells are subsets of T lymphocytes that help in innate immune response because they can be directly cytotoxic. Our study was performed to investigate the role of NK cells and NKT cells in the pathogenesis of HCV infection. Our study was conducted on 30 adult patients (18 males and 12 females) with chronic HCV infections. All patients were seropositive for HCV antibodies and positive for HCV-RNA. Anti– CD56, Anti– Cd3, and Anti–perforine labeled monoclonal antibodies were used for flow cytometry. There was a significant reduction in the frequency and function of the NK cells (CD3- and CD56+) coincided with a quantitative imbalance of the CD56bright and CD56dim Subsets within the total NK population. Also, there was a shift in NK subsets dim into bright with a marked decrease in the CD56dim cell fraction as compared to CD56bright cells. A decrease in the frequency and function of the NKT cells (CD3+ and CD56+) in the peripheral blood of chronic HCV infected patients, compared to healthy controls, was observed. NK and NKT cells play important roles in the pathogenesis of chronic hepatitis C virus infection.


Keywords: NK cells, NKT cells, Chronic HCV infection, Innate immune response in chronic HCV infection.
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How to cite this article:

Marwa Mostafa Shalaby, Kareman Ahmed Eshra, Radwa Mahmoud Sharaby and Ibrahim Fathy Amer. 2017. Flowcytometric Detection of Natural Killer Cells and Natural Killer T Cells Changes in the Blood of Patients with Chronic Hepatitis C Virus Infection in an Egyptian University Hospital.Int.J.Curr.Microbiol.App.Sci. 6(3): 1506-1518. doi: https://doi.org/10.20546/ijcmas.2017.603.173