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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1461-1467
DOI: https://doi.org/10.20546/ijcmas.2017.603.167


Characterization of VIM and IMP Metallobetalactamases (MBL) in Pseudomonas aeruginosa Isolated in a Tertiary Care Hospital
R. Mahesh Reddy*, Satish Kumar and Mahadevan Kumar
Department of Microbiology, Armed Forces Medical College, Pune – 411048, Maharashtra, India
*Corresponding author
Abstract:

Resistance to carbapenems are because of various mechanisms like mutations in the outer membrane proteins like Opr D causing decreased permeability of the drugs, the carbapenem hydrolyzing enzymes – carbapenemases and the efflux mechanisms. Carbapenem resistance was studied in 140 non-repeat clinical isolates of Pseudomonas aeruginosa in a tertiary care hospital. Imipenem resistance was detected using Kirby Bauer’s standard disc diffusion method. MIC for Imipenem was detected in all resistant isolates using E-test strips. Production of Metallo-betalactamases (MBL) was detected using phenotypic MBL E-test method. MBL producing genes were tested in Pseudomonas using conventional PCR for IMP, VIM-type genes. Out of 140 isolates, 68 (48.5 %) showed imipenem resistance. MIC in majority (58 %) of imipenem resistant isolates was ≥ 32µg/ml. MBL E-test, showed 54 (79.4%) as positive for MBL. The PCR analysis of the 54 isolates which were detected to have MBL showed VIM type (43 %), IMP-1 (12%), IMP-2 (2%) like genes. There is high level of prevalence of imipenem resistance in the Pseudomonas aeruginosa strains isolated. The carbapenem resistance in Pseudomonas aeruginosa in our hospital setting is mainly because of MBL gene that is of VIM type.


Keywords: Carbapenem resistance, Pseudomonas aeruginosa, Metallo-betalactamases (MBL), MBL genes.
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How to cite this article:

Mahesh Reddy, R., Satish Kumar and Mahadevan Kumar. 2017. Characterization of VIM and IMP Metallobetalactamases (MBL) in Pseudomonas aeruginosa Isolated in a Tertiary Care Hospital.Int.J.Curr.Microbiol.App.Sci. 6(3): 1461-1467. doi: https://doi.org/10.20546/ijcmas.2017.603.167