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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1355-1363
DOI: https://doi.org/10.20546/ijcmas.2017.603.157


Quantitative Screening of Lignocellulose Degrading Fungi Using Digested Biogas Slurry as a Substrate
Ajit Kaur1 and Urmila Gupta Phutela2*
1Department of Biofuels, Centre of Excellence in Farm Machinery,CSIR-CMERI, Ludhiana-141006, India
2School of Renewable Energy Engineering, Punjab Agricultural University,Ludhiana-141004, Punjab, India
*Corresponding author
Abstract:

Digested biogas slurry is a rich lignocellulosic substrate, consistent with the idea of sustainable agriculture and development. This work is aimed at utilization of digested biogas slurry as a substrate for the quantitative screening of lignocellulose degrading fungi. In this study, the potential of digested biogas slurry as a substrate to determine the lignocellulolytic enzyme production after 8 days of incubation at 30±2ºC (mesophilic) and 50±2ºC (thermophilic) was investigated. Standard mesophilic culture Phanaerochaete chrysosporium MTCC 787 showed maximum enzyme activities (3.9U/ml endoglucanase, 2.09 U/ml exoglucanase,  29.45 U/ml β-glucosidase, 55 U/ml xylanase, 69.5 U/ml mannanase, 48.33U/ml lignin peroxidase, 13.3 U/ml Manganese peroxidase) followed by isolate A5, A4, A12.  Among thermophilic cultures, isolate A31 is the best cellulolytic fungal culture. Thermoascus aurantiacus (1.97U/ml endoglucanase), isolate A25 (23.08U/ml β-glucosidase, 2.35U/ml exoglucanase, 63U/ml Mannanase) and A31 (48.08 U/ml xylanase, 11.5 U/ml lignin peroxidase, 5.6U/ml laccase) are thus the best lignolytic fungal cultures. The results show that digested biogas slurry, which otherwise is considered as a waste of biogas plants, can be efficiently utilized to screen different fungi for their lignocelluloses degrading potential. Mesophilic cultures Phanaerochaete chrysosporium, isolate A4 and A5 and thermophilic cultures Thermoascus aurantiacus, isolate A25 and A31 are the best lignocelluloses degraders.


Keywords: Fungi, Digested biogas slurry, Enzymes, Biodegradation, Waste utilization
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How to cite this article:

Ajit Kaur and Urmila Gupta Phutela. 2017. Quantitative Screening of Lignocellulose Degrading Fungi Using Digested Biogas Slurry as a SubstrateInt.J.Curr.Microbiol.App.Sci. 6(3): 1355-1363. doi: https://doi.org/10.20546/ijcmas.2017.603.157