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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3346-3355
DOI: https://doi.org/10.20546/ijcmas.2020.905.398


Assessment of Genetic Diversity in Rice Germplasm (Oryza sativa L.) using SSR Markers
Akhand Pratap1, Prashant Bisen2*, Bapsila Loitongbam3, Sanket R. Rathi1,Pragya Parmita4, B. P. Singh5 and P. K. Singh1*
1Department of Genetics and Plant Breeding, Institute of Agricultural Sciences,
Banaras Hindu University, Varanasi- 221005, U. P., India
2Narayan Institute of Agricultural Sciences, Gopal Narayan Singh University,
Jamuhar, Rohtas- 821305, Bihar, India
3Department of Genetics and Plant Breeding, College of Agriculture,
Central Agriculture University (Imphal), Pasighat, Arunachal Pradesh, 791102, India
4Department of Genetics and Plant Breeding,
Udai Pratap Autonomous College, Varanasi, U.P.- 221003, India
5State Agriculture Management Institute, Rahmankhera, Lucknow, U.P., India
*Corresponding author
Abstract:

Thirty eight rice accessions were analyzed to evaluate the genetic polymorphism and identification of diverse parents using simple sequences repeat (SSR) markers. These accessions showed significant phenotypic variation for all the characters studied. A total of 61 alleles were detected by 11 polymorphic markers showing highly polymorphic across all accessions with an average of 5.54 alleles per polymorphic marker. Five of the markers RM324, RM171, RM31, RM24, RM30 produced maximum 6 alleles. The PIC value ranged from 0.275 to 0.751 with a mean PIC of 0.633 and Markers RM31, RM336, RM30 and RM280 were the most informative primers to discriminate among the accessions on the basis of highest PIC of 0.751, 0.735, 0.731 and 0.719, respectively. The cluster analysis showed that these accessions grouped in to seven clusters in which cluster IIB had maximum 8 genotypes followed by cluster IA-1 (7 germplasms) and cluster IA-2 (6 germplasms). While, highest dissimilarity coefficient value was observed between the cultivar cultivar C-3 and LAL KHADHA. These accessions were showing wide genetic divergence among the constituent in it and may be directly utilized in hybridization programme for improvement of yield related traits.


Keywords: Genetic polymorphism,Diverse parents,Simple sequences repeat
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How to cite this article:

Akhand Pratap, Prashant Bisen, Bapsila Loitongbam, Sanket R. Rathi, Pragya Parmita, B. P. Singh and Singh, P. K. 2020. Assessment of Genetic Diversity in Rice Germplasm (Oryza sativa L.) using SSR Markers.Int.J.Curr.Microbiol.App.Sci. 9(5): 3346-3355. doi: https://doi.org/10.20546/ijcmas.2020.905.398