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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3183-3191
DOI: https://doi.org/10.20546/ijcmas.2020.905.378


Amylase and Biosurfactant Production from Bacillus Species
Olusegun Richard Adeoyo*
Department of Microbiology, Adekunle Ajasin University, P.M.B. 001,
Akungba-Akoko, Ondo State, Nigeria
*Corresponding author
Abstract:

Most Bacillus species have ability to produce secondary metabolites such as amylases and biosurfactants. This study was carried out to determine the amylase and boisurfactant production potentials of some Bacillus species obtained from agricultural soil. Soil samples were collected from agricultural research farm of Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria. All isolates were identified based on the morphological and biochemical characteristics using Bergey’s manual of determinative bacteriology. Amylase-producing Bacillus species were selected and screened on soluble starch agar while biosurfactant activity was determined by three methods; blood haemolysis, oil spreading, and drop-collapse tests. The extent of amylase activity of each isolate was determined quantitatively using dinitrosalicylic acid (DNSA) assay method. A total of ten (10) Bacillus species were isolated and identified from the samples. These isolates include two B. subtilis (AMY5 and AMY13), B. mycoides, two B. polymyxa (AMY2 and AMY12), B. azotoformans B. licheniformis, B. megaterium, B. pumilus and B. cereus. The enzyme assay results indicated that B. megaterium (Isolate code: AMY17) had the best activity value of 1.12 𝜇mol/min/mL while B. cereus had the least value 0.27 𝜇mol/min/mL. All Bacillus species demonstrated great potentials for biosurfactant production except B. subtilis (AMY10). Overall, B. licheniformis (AMY17) and B. subtilis (AMY5) had the highest biosurfactant activity. Therefore, Bacillus species are promising sources of amylases and biosurfactants for the bioeconomy and their use should be encouraged.


Keywords: Amylases, Biosurfactants, Agricultural soil, Bacillus species
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How to cite this article:

Olusegun Richard Adeoyo. 2020. Amylase and Biosurfactant Production from Bacillus Species.Int.J.Curr.Microbiol.App.Sci. 9(5): 3183-3191. doi: https://doi.org/10.20546/ijcmas.2020.905.378