National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Most Bacillus species have ability to produce secondary metabolites such as amylases and biosurfactants. This study was carried out to determine the amylase and boisurfactant production potentials of some Bacillus species obtained from agricultural soil. Soil samples were collected from agricultural research farm of Adekunle Ajasin University, Akungba-Akoko, Ondo State, Nigeria. All isolates were identified based on the morphological and biochemical characteristics using Bergey’s manual of determinative bacteriology. Amylase-producing Bacillus species were selected and screened on soluble starch agar while biosurfactant activity was determined by three methods; blood haemolysis, oil spreading, and drop-collapse tests. The extent of amylase activity of each isolate was determined quantitatively using dinitrosalicylic acid (DNSA) assay method. A total of ten (10) Bacillus species were isolated and identified from the samples. These isolates include two B. subtilis (AMY5 and AMY13), B. mycoides, two B. polymyxa (AMY2 and AMY12), B. azotoformans B. licheniformis, B. megaterium, B. pumilus and B. cereus. The enzyme assay results indicated that B. megaterium (Isolate code: AMY17) had the best activity value of 1.12 ðœ‡mol/min/mL while B. cereus had the least value 0.27 ðœ‡mol/min/mL. All Bacillus species demonstrated great potentials for biosurfactant production except B. subtilis (AMY10). Overall, B. licheniformis (AMY17) and B. subtilis (AMY5) had the highest biosurfactant activity. Therefore, Bacillus species are promising sources of amylases and biosurfactants for the bioeconomy and their use should be encouraged.
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