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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3161-3167
DOI: https://doi.org/10.20546/ijcmas.2020.905.375


In vitro Root Architectural Screening for Early Drought Adaptive Traits in Sorghum (Sorghum bicolor L.)
Williams Mohanavel1, N. Manikanda Boopathi1*,Raveendran Muthurajan1 and A. Senthil2
1Department of Plant Biotechnology, Center for Plant Molecular Biology and Biotechnology, 2Department of Crop Physiology, Tamil Nadu Agricultural University,Coimbatore 641003, India
*Corresponding author
Abstract:

Drought is a major abiotic stress that causes a great reduction in the yield of the key cereal crops including sorghum. As the crops are sessile, they evolved several drought tolerance component traits to combat the water stress. Among the drought adaptive traits, root architecture plays a major role in efficient capillary water absorption even under severe water scarce situations through early development and deep penetration of roots. Thus, introgression of root traits in the elite breeding lines would enhance the breeding efficiency for water-limited environments. On the other hand, when compared with other drought tolerance component traits, phenotyping of root architecture is extremely challenging as it involves extensive excavation and destructive methodologies. Hence, a simple and efficient in vitro root architectural phenotyping in sorghum has been proposed in this study. Surface sterilization of sorghum seeds with 0.1 % HgCl2 and subsequent inoculation of the seeds in ½ MS media provided effective visualization of root development and further analysis of root architecture traits. Such effort has helped to assess the significant genetic variation among ten different germplasm accessions used in this investigation. We suggest that such an in vitro screening methodology which is utilized to capture initial root development can be used as a rapid, non-destructive and efficient high-throughput tool to select the early drought adaptive lines among the large numbers of plant breeding materials.


Keywords: Root in-vitro study, Root architecture, Large scale phenotypic screening, Sorghum
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How to cite this article:

Williams Mohanavel, N. Manikanda Boopathi, Raveendran Muthurajan and Senthil, A. 2020. In vitro Root Architectural Screening for Early Drought Adaptive Traits in Sorghum (Sorghum bicolor L.).Int.J.Curr.Microbiol.App.Sci. 9(5): 3161-3167. doi: https://doi.org/10.20546/ijcmas.2020.905.375