National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Drought is a major abiotic stress that causes a great reduction in the yield of the key cereal crops including sorghum. As the crops are sessile, they evolved several drought tolerance component traits to combat the water stress. Among the drought adaptive traits, root architecture plays a major role in efficient capillary water absorption even under severe water scarce situations through early development and deep penetration of roots. Thus, introgression of root traits in the elite breeding lines would enhance the breeding efficiency for water-limited environments. On the other hand, when compared with other drought tolerance component traits, phenotyping of root architecture is extremely challenging as it involves extensive excavation and destructive methodologies. Hence, a simple and efficient in vitro root architectural phenotyping in sorghum has been proposed in this study. Surface sterilization of sorghum seeds with 0.1 % HgCl2 and subsequent inoculation of the seeds in ½ MS media provided effective visualization of root development and further analysis of root architecture traits. Such effort has helped to assess the significant genetic variation among ten different germplasm accessions used in this investigation. We suggest that such an in vitro screening methodology which is utilized to capture initial root development can be used as a rapid, non-destructive and efficient high-throughput tool to select the early drought adaptive lines among the large numbers of plant breeding materials.
Citations |
 |
 |
 |
 |
