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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 3124-3133
DOI: https://doi.org/10.20546/ijcmas.2020.905.370


Antifungal Activity of New Bacterial Biocontrol Agents against Diplocarpon rosae Causing Black Spot Disease of Rose
Shalini1, M. Jayasekhar2*, K. G. Sabarinathan1, R. Akila1 and R. Kannan1
1Department of Plant Pathology, Agrl. College & Res. Institute,
TNAU, Killikulam-628252, India
2Agricultural Research Station, Tamil Nadu Agricultural University,
Thirupathisaram-629901, India
*Corresponding author
Abstract:

The phylloplane and rhizosphere microbes of rose cv. Edward was isolated and nine bacteria were selected to observe their antagonistic efficacy against Diplocarpon rosae causing blackspot disease in rose. The per cent inhibition of mycelial growth of the fungi by bacterial isolates was observed. The bacterial isolates PB1 and PB2 recorded 100 per cent inhibition followed by SB1, PB2 and SB2 isolates. After molecular characterization the isolates PB1 and PB3 were found to be Pseudomonas aeruginosa and it causes life threatening infections in human beings however, the isolates SB1, SB2 and PB2 were identified as Bacillus subtilis, Brevibacillus sp., Lysinibacillus fusiformis respectively. In the field experiments the native isolate Brevibacillus sp. (SB2) was highly effective in reducing the leaf spot disease incidence by 42.94 and 33.39 per cent in Kashmir rose and Edward rose varieties with C:B ratio of 1:2.69 and 1:2.82 respectively followed by L.fusiformis (PB2) with 38.26 and 31.07 per cent reduction in disease incidence by reducing the defoliation with a C:B ratio of 1:2.60 and 1:2.59. This study indicated that the new bacterial isolates isolated from rhizosphere (Brevibacillus sp.) and phylloplane (L.fusiformis) of rose cv. Edward have the potential to produce antifungal compounds which can be used to control the black leaf spot disease of rose caused by D.rosae.


Keywords: Rose, Black spot, Biological control, Brevibacillus sp., Lysinibacillus fusiformis
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How to cite this article:

Shalini, M. Jayasekhar, K. G. Sabarinathan, R. Akila and Kannan, R. 2020. Antifungal Activity of New Bacterial Biocontrol Agents against Diplocarpon rosae Causing Black Spot Disease of Rose.Int.J.Curr.Microbiol.App.Sci. 9(5): 3124-3133. doi: https://doi.org/10.20546/ijcmas.2020.905.370