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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 2630-2637
DOI: https://doi.org/10.20546/ijcmas.2020.905.301


Evaluation of the Effect of GnRH Analogue, Progesterone and
Tolfenamic Acid on Serum Progesterone Profile and Conception
Rate in Repeat Breeding Crossbred Cattle
Sudhanshu Pratap Singh1*, Ankesh Kumar2, Prakrutik Prafulchandra Bhavsar3, Mukesh Sahu4, Praveen Kumar1 and Sushil Kumar5
1Department of Veterinary Gynaecology and Obstetrics, 2Department of Veterinary Clinical Complex, 5Department of Veterinary Medicine, Bihar Animal Sciences University, Patna, Bihar, India 3College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, India 4Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Animal Sciences, G. B. Pant University of Agriculture and Technology, Pant Nagar, Uttarakhand, India
*Corresponding author
Abstract:

Secretion of prostaglandin F at the time of maternal recognition of pregnancy leads to embryonic loss. Early embryonic losses are one of the important causes of repeat breeding which increases the calving interval and leads to economic losses to farmers. Non-steroidal anti-inflammatory drugs inhibit the synthesis of PGF. The present study was designed to study the effect of treatment by GnRH analogue - Buserelin acetate, Progesterone (P4) and Tolfenamic acid on 32 repeat-breeding crossbred cattle aged 3-8 years. All the animals were randomly divided into 4 equal groups (n=8). Group 1 was taken as positive control i.e. inseminated on spontaneous estrous without any therapy. Group 2 animals were treated with injection Buserelin acetate @ 20 µg intramuscular (IM) at the time of artificial insemination (AI). Group 3 animals were treated with inj. Buserelin acetate @ 20 µg intramuscular at the time of AI followed by Injection P4 @100mg IM on days 4, 5, 6 after AI. Group 4 animals were treated with inj. Buserelin acetate @ 20 µg IM at the time of AI, inj. P4 @ 100mg IM on days 4, 5, 6 and inj. Tolfenamic acid @4 mg/kg body weight IM on days 16, 17, 18 after AI. Blood samples were collected from all the cows at day 0, 7, 16, 17, 18 of the estrous cycle for the study of serum progesterone profile. The conception rate observed in groups 1, 2, 3 and 4 were 12.5%, 37.5%, 50% and 75% respectively. Highest conception was observed in group 4 in which, combinations of Buserelin acetate, Exogenous P4 and Tolfenamic acid was administered. The study concluded that combination of Buserelin acetate, Exogenous P4 and Tolfenamic acid therapy helps to maintain the P4 level and significantly increases the conception rate by 6 times than control group.


Keywords: Repeat breeders, Progesterone, Tolfenamic acid, Conception rate
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How to cite this article:

Sudhanshu Pratap Singh, Ankesh Kumar, Prakrutik Prafulchandra Bhavsar, Mukesh Sahu, Praveen Kumar and Sushil Kumar. 2020. Evaluation of the Effect of GnRH Analogue, Progesterone and Tolfenamic Acid on Serum Progesterone Profile and Conception Rate in Repeat Breeding Crossbred Cattle.Int.J.Curr.Microbiol.App.Sci. 9(5): 2630-2637. doi: https://doi.org/10.20546/ijcmas.2020.905.301