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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020]For more details click here

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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 2320-2338
DOI: https://doi.org/10.20546/ijcmas.2020.905.265


Immunological Evaluation of Fc Receptor Mediated Antigen Delivery of Brucella melitensis Recombinant Outer Membrane Protein 28 (Romp 28) in Mice
Ramya Kalaivanan1*, Sankar Palanisamy1, Tapas Kumar Goswami2, Pallab Chaudhury2 and G. C. Ram2
1Veterinary College and Research Institute, Namakkal, Tamil Nadu Veterinary and Animal Sciences University, Chennai, India
2Indian Veterinary Research Institute, Izatnagar Bareilly, India
*Corresponding author
Abstract:

The present study was aimed to prepare a conjugate of Fc fragment of mice IgG with rOMP 28 of Brucella melitensis and investigate its ability to influence the kinetics of humoral and cellular immune response in comparison to its unconjugated counterpart and the rOMP 28 alone in the mice model. Among all the groups, the group of mice immunized with rOmp28 alone induced highest level of humoral immune response and the vaccine strain group(S19) conferred highest level of cellular response which was evidenced by higher nitric oxide concentration and the clearance of virulent B. abortus 544 from the spleen upon challenge infection. The conjugated group conferred low level of humoral response compared to the rOMP28 alone which may be due to the involvement of inhibitory FcγIIR in the regulation of immune responses, soluble forms of FcR inhibiting antigen presentation and irreversible loss of Fc receptors on the macrophage plasma membrane. The S19 strain of B. abortus, a live vaccine conferred high level of protection when challenged with virulent B. abortus 544which indicate whole cell organism composed of various immunogenic components might have been involved in inducing protection.


Keywords: Outer membrane protein (rOMP 28), Fc fragment of IgG, SDS PAGE, Protection, Challenge infection
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How to cite this article:

Ramya Kalaivanan, Sankar Palanisamy, Tapas Kumar Goswami, Pallab Chaudhury and Ram, G. C. 2020. Immunological Evaluation of Fc Receptor Mediated Antigen Delivery of Brucella melitensis Recombinant Outer Membrane Protein 28 (Romp 28) in Mice.Int.J.Curr.Microbiol.App.Sci. 9(5): 2320-2338. doi: https://doi.org/10.20546/ijcmas.2020.905.265