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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 2266-2275
DOI: https://doi.org/10.20546/ijcmas.2020.905.258


In vitro Comparative Efficacy of Various Concentrations of Aqueous and Methanolic Leaf Extract of Ocimum sanctum against Tetranychus urticae Koch in Tomato
Sonika Khatak*, Rachna Gulati, Monika Jangra and Anju
Department of Zoology and Aquaculture, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004, India
*Corresponding author
Abstract:

In present investigation, the bioefficacy of aqueous and methanolic leaf extracts of Ocimum sanctum were tested to evaluate their toxic effects at different concentration (10.00, 7.50, 5.00, 2.50, 2.00, 1.50, 1.00 and 0.50 percent) against mixed population of Tetranychus urticae on tomato during 2018 under in vitro conditions. T. urticae responded to the mite in a concentration dependent manner i.e. lowest number of live mites and highest mortality in population was obtained with highest concentration tested (10.0%). Out of an initial number 10 mite, significantly less number of mites were recorded at 10.00 percent concentration of O. sanctum leaf extract followed by 7.50, 5.00, 2.50 and 2.50 per cent concentration than 1.50, 1.00 and 0.50 percent treatment and control. Direct spray bioassay results clearly revealed that aqueous and methanolic leaf extract of O. sanctum possessed acaricidal activity. Mites responded to extract in a concentration dependent manner. The methanolic leaf extract was more potent to T. urticae causing 20 to 71.9 percent mortality at 0.5 to 10 percent concentration as compared to aqueous leaf extract (6.7 to 61% mortality). The LC50 value in methanol extract was 3.46 percent while it was 7.06 percent in aqueous extract against T. urticae.


Keywords: Leaf extract, Mites, Ocimum sanctum, Tetranychus urticae
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How to cite this article:

Sonika Khatak, Rachna Gulati, Monika Jangra and Anju. 2020. In vitro Comparative Efficacy of Various Concentrations of Aqueous and Methanolic Leaf Extract of Ocimum sanctum against Tetranychus urticae Koch in Tomato.Int.J.Curr.Microbiol.App.Sci. 9(5): 2266-2275. doi: https://doi.org/10.20546/ijcmas.2020.905.258