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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1691-1700
DOI: https://doi.org/10.20546/ijcmas.2020.905.190


Effect of Composite Culture of Azotobacter and Phosphate Solubulizing Bacteria on in vitro Propagation of Musa acuminate (Banana)
Eurendra Kumar1, L. S. Verma2*, D. Dash1 and S. B. Gupta1
1Department of Agricultural Microbiology, 2Department of Floriculture and Landscape Architecture, College of Agriculture, IndiraGandhi Krishi Vishwavidyalaya, Raipur 492012 (Chhattisgarh), India
*Corresponding author
Abstract:

The present study was conducted to assess the effect of composite culture of Azotobacter and Phosphate solubulizing bacteria with different combinations of organic, inorganic fertilizations on growth performance of in vitro propagated Banana, cv. Grand Naine. The in vitro rooted plantlets were hardened and acclimatized by using different treatments (five treatments comprising of inoculation of composite culture, vermicompost and Inorganic fertilization) carried out in CRD replicated four times. Propagation studies on primary hardening showed cocopeat was the best medium for primary hardening in terms of percentage survival of plantlets (100.00 %). Plantlets showed significantly highest height (9.50 cm), leaf number (2.80 per plantlet), girth (4.50 mm) when inoculation was given along with 25%NPK (organic) + 75% NPK (inorganic) in primary hardening. Similarly during secondary hardening, inoculation + 25%NPK (organic) + 75% NPK (inorganic) recorded best results in terms of plantlet height (20.45 cm), plantlet diameter (11.60cm) no. of leaves (5.25), length of primary roots per plantlet (12.32 cm). Maximum survival during secondary hardening (100.00 %) with composite culture inoculation whereas in other treatments survival percentage ranged from 87.5-93.5%. Nitrogen uptake by banana significantly increased from 9.17 at control to 28.21 mg per plantlet at inoculation + 25%NPK (organic) + 75% NPK (inorganic). For hardening and acclimatization of in vitro propagated banana, cv Grand Naine, composite culture + 25%NPK (organic) + 75% NPK (inorganic) showed overall superiority being the best treatment.


Keywords: Composite culture, Azotobacter, Musa acuminate, NPK, Vermicompost
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How to cite this article:

Eurendra Kumar, L. S. Verma, D. Dash and Gupta, S. B. 2020. Effect of Composite Culture of Azotobacter and Phosphate Solubulizing Bacteria on in vitro Propagation of Musa acuminate (Banana).Int.J.Curr.Microbiol.App.Sci. 9(5): 1691-1700. doi: https://doi.org/10.20546/ijcmas.2020.905.190