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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1974-1987
DOI: https://doi.org/10.20546/ijcmas.2020.905.224


Comparative Assessment of Endoglucanase Activity of Cellulolytic Bacterial Strains on Untreated and Pretreated Natural Cellulosic Wastes
Pinky Prasad1*, Tanuja2 and Sheila Bedi1
1Department of Botany, Patna Women’s College, Patna University, Patna, Bihar, India
2Department of Botany, T. P. S. College, Patna, Pataliputra University, Bihar, India
*Corresponding author
Abstract:

The aim of the research was isolation of efficient cellulolytic bacterial strains from soil samples and comparative assessment of their cellulolytic potential on untreated and pre-treated cellulosic wastes namely sugarcane bagasse, groundnut shell, corn cob, and vegetable peel so as to determine a suitable substrate pre-treatment for cellulolytic enzyme production. The substrates were pretreated with 0.5% NaOH (alkali treatment) and 0.5% HCl (acid treatment) and the endoglucanase enzyme production was analyzed on untreated, alkali treated and acid treated substrates by measuring the amount of glucose liberated in IU/ ml by using the dinitrosalicylic acid assay method. A total of 22 cellulose degrading bacterial strains isolated from 10 soil samples collected randomly from agricultural fields and municipal wastes from different locations in Patna region were screened after primary screening using Congo red and out of these, six of the most promising isolates labeled as PCD1, PCD2, PCD3, PCD4, PCD5 and PCD6 were selected for further investigations on the basis of their enzymatic activity. Amplified and sequenced portion of the 16S rRNA genes of these isolates were BLASTed to determine likely genera and are deposited in the GenBank with their Accession Numbers. PCD1 was identified as Brevibacillus brevis (Accession No. KP851193), PCD2 as Streptomyces matensis (Accession No. KP890933), PCD3 as Ochrobactrum intermedium (Accession No. KP851194), PCD4 as Bacillus subtilis (Accession No. KP851195), PCD5 as Streptomyces griseorubens (Accession No. KP851196) and PCD6 as Achromobacter xylosoxidans (Accession No. KP851197). Comparing the enzyme production by all the selected strains on all the specified untreated and pretreated cellulosic wastes, it was concluded that the maximum amount of enzyme production (0.2427 IU/ml) was observed on alkali treated sugarcane bagasse by the strain Bacillus subtilis.


Keywords: Natural cellulosic wastes, Cellulolytic bacterial strains, Endoglucanase activity, Exoglucanase activity
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How to cite this article:

Pinky Prasad, Tanuja and Sheila Bedi. 2020. Comparative Assessment of Endoglucanase Activity of Cellulolytic Bacterial Strains on Untreated and Pretreated Natural Cellulosic Wastes.Int.J.Curr.Microbiol.App.Sci. 9(5): 1974-1987. doi: https://doi.org/10.20546/ijcmas.2020.905.224