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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 1814-1818
DOI: https://doi.org/10.20546/ijcmas.2020.905.206


Estrus Induction by Hormonal and Nutritional Approaches in Canines
Honparkhe1, Jugraj Singh Mahal2* and Ajeet Kumar1
1Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India
2Department of veterinary Gynaecology and Obstetrics, Khalsa College of Veterinary and Animal sciences, Amritsar, Punjab, India
*Corresponding author
Abstract:

The present study was conducted to compare the efficacy of hormonal and nutritional approaches to induce fertile estrus in canine. Twenty female dogs of different breeds presented to university clinics, GADVASU, Ludhiana were subjected to hormonal (Group 1, n=10) and nutritional (Group 2; n=10) treatments. The breeds of these dogs were Labrador, Dalmatian and German Shephard weighing between 25-30 kg with the history of not showing estrus despite attaining age of puberty (≥ 1.5 years of age). Prior to start of any treatment, all the animals were subjected to deworming and transabdominal ultrasonography of reproductive organ to assess uterine development. Hormonal treatment included eCG @ 5 IU/kg (Inj Folligon; MSD Intervet) daily for 10 days followed by hCG 500 IU (Inj Chorulon, MSD Intervet) intramuscularly on day 11. Nutritional treatment involved oral administration of tab Nutrich (Virbac, India) @ one tab daily for 15 days and tab Evion LC (Merk India) @ 1 tab daily for 15 days one month before start of breeding season. Following hormonal treatment majority of Group 1 female dogs (7/10) exhibited proestrus bleeding at 5-6 days of eCG treatment which was confirmed by exfoliative vaginal cytology indicating abundant parabasal cells, few large and small intermediate cells and RBCs. On day 11, these animals were again subjected to vaginal cytology to confirm presence of abundant superficial cells (>80%). These dogs were advised for mating twice at 24 and 48 hrs after hCG administration. Two female dogs from group 1 encountered with the problem of ovarian cysts whereas one had developed cystic endometrial hyperplasia. Group 2 animals exhibited proestrus bleeding (5/10) during subsequent breeding season and were subjected to vaginal cytology twice (i.e. first at 4 days after start of bleeding and second next day after the end of bleeding). These animals were advised for mating when >80% superficial cells were observed in vaginal cytology. Following treatment, five and four animals conceived respectively in groups 1and 2. The results indicated that hormonal and nutritional approaches are equally good in inducing fertile estrus in canine.


Keywords: Hormonal and nutritional Treatments,Transabdominal ultrasonography
 
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How to cite this article:

Honparkhe, M., Jugraj Singh Mahal and Ajeet Kumar. 2020. Estrus Induction by Hormonal and Nutritional Approaches in Canines.Int.J.Curr.Microbiol.App.Sci. 9(5): 1814-1818. doi: https://doi.org/10.20546/ijcmas.2020.905.206