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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 853-863
DOI: https://doi.org/10.20546/ijcmas.2020.905.095


Immunogenicity of Wild Strain of Duck Plague Virus Adapted in Chicken Embryo Fibrolast
Nayanmoni Konwar1, Kevisenuo E. Vizo1, Sophia M. Gogoi1, Barnali Talukdar1
Mamta Pathak2,3, Nagendra N. Barman1, Sailendra K. Dasand Durlav P. Bora1*
1Department of Microbiology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022, India
2Department of Pathology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781 022
3Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, India
*Corresponding author
Abstract:

Duck plague is an acute, fatal viral infection of ducks, geese, swans and other species of the order Anseriformes. The present study was undertaken for studying the immunogenicity of a wild strain of Duck Plague virus (DPV) which was adapted to grow in Chicken embryo fibroblast (CEF) cells. For this study a wild strain of DPV (DP/As-Km/0019) available in the Department of Microbiology was selected on the basis of its virulence and nucleotide homology with the vaccine strain. The selected wild strain was passaged in Chicken embryo fibroblast cell culture up to the12th passage and its immunogenicity was studied. Two groups (Group I & II) of ducklings were taken with 6 numbers of ducklings in each group. Group I was kept as unvaccinated control while Group II was used for vaccination. Significant rise in the antibody titres could be observed in Group II ducklings without producing any adverse effects. The mean antibody titres in vaccinated birds on 7th day were 16.66±2.108 with an increasing trend to 60±8.944 on 21st day and 133.33±16.865 on 30th day post vaccination respectively.


Keywords: Duck plague virus, Chicken embryo fibroblast, Polymerase chain reaction ,Immunogenicity trial, Neutralization
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How to cite this article:

Nayanmoni Konwar, Kevisenuo E. Vizo, Sophia M. Gogoi, Barnali Talukdar, Mamta Pathak, Nagendra N. Barman, Sailendra K. Das and Durlav P. Bora. 2020. Immunogenicity of Wild Strain of Duck Plague Virus Adapted in Chicken Embryo Fibrolast.Int.J.Curr.Microbiol.App.Sci. 9(5): 853-863. doi: https://doi.org/10.20546/ijcmas.2020.905.095