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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2019: 96.39
Index Copernicus ICI Journals Master List 2019 - IJCMAS--ICV 2019: 96.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 818-828
DOI: https://doi.org/10.20546/ijcmas.2020.905.091


Morphological Characterization and Genetic Diversity of Fusarium spp. Infecting Bitter Gourd
M. Saran Kumar1*, S. K. Manoranjitham1, V. Sendhilvel1 and V. Rajasree2
1Department of Plant Pathology, Tamil Nadu Agricultural University,Coimbatore – 641 003, India
2Department of Vegetable Science, Tamil Nadu Agricultural University,Coimbatore – 641 003, India
*Corresponding author
Abstract:

Bitter gourd (Momordica charantiaL.) is an important cucurbitaceous plant in which the deleterious diseases namely vascular wilt, damping off occurs commonly and causes 30 to 50 percent losses in crop. The present investigation was aimed to study the morphological and molecular characterization of disease causing pathogen to understand the etiology of the disease.Survey was conducted at different locations of Coimbatore, Erode and Dharmapuri and samples were collected for the isolation of the pathogen. The pathogen was isolated from the brownish discoloured vascular tissue of stem portion which was typically wilted. Pathogenicity assay was proved and the virulence of the fusarium isolates were identified under artificial inoculation studies. The morphological studies of Fusarium isolates were studied using the microscopic observation to study the micro and macro conidial variation.The molecular confirmation of pathogen was done by PCR amplification using ITS 1 and ITS 4primer at ̴̴ 560 bp. The different species of Fusarium solani, Fusarium equiseti, Fusarium falciforme, Fusarium chlamydosporum and Fusarium incarnatumwere identified in these molecular studies. Among the species, Fusarium solani is a major pathogen associated with bitter gourd wilt disease. The genetic variability studies among different Fusariumspp. was carried out using RAPD marker. The variability studies revealed that virulence isolates viz, F. solani VP, F. solani EL, F. solani TP, F. falciforme IJ and F. chlamydosporum TN were grouped into cluster II which are more virulent and the same results were shown in pathogenicity. The wilt pathogen of bitter gourd isolated from the palee hybrid was shown more virulence when compared to the other isolates.


Keywords: Bitter gourd, Fusarium spp., Pathogenicity, PCR amplification, Genetic diversity.
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How to cite this article:

Saran Kumar. M., S. K. Manoranjitham, V. Sendhilvel and Rajasree. V. 2020. Morphological Characterization and Genetic Diversity of Fusarium spp. Infecting Bitter Gourd.Int.J.Curr.Microbiol.App.Sci. 9(5): 818-828. doi: https://doi.org/10.20546/ijcmas.2020.905.091