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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(5): 415-426
DOI: https://doi.org/10.20546/ijcmas.2020.905.046


Isolation and Identification of Bacillus Species from Soil for Phosphate, Potassium Solubilisation and Amylase Production
Shambhavi1, Ravindra Kumar1*, Akash Tomar1, Purushottam1, Jitender Singh1 and Shishu Pal Singh2
1College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110
2College of Agriculture, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, Uttar Pradesh, India-250110
*Corresponding author
Abstract:

In the present study ten different Bacillus isolates were isolated from various soil samples and their identification was performed on the basis of Gram staining, colony morphology and biochemical tests. Gram positive rod shaped bacteria showing large, circular, opaque colonies having white or greyish white appearance were preserved on Nutrient agar slants for further identification. All isolates showed positive results for Catalase, Urease, Oxidase and motility biochemical tests. For other biochemical tests such as Citrate utilization, Vogues Proskauer, Nitrate reduction, Triple sugar iron Agar test, mixed results were obtained. Phosphate solubilisation and Potassium solubilisation activities of different isolates was performed. Out of ten isolates, none of the isolates solubilised phosphate on Pikovskaya’s agar medium at 30°C after 15 days of incubation. Potassium solubilising activity was shown by two isolates (Sample code 3 and 9) out of ten. Among these two isolates, Sample code 3 showed highest potassium solubilisation and the clear zone on Aleksandrov agar medium was 9 mm and that of Sample Code 9 was 5 mm, after 10 days of incubation at 30°C. Further, the isolates were screened for the production of amylase and its assay has been performed. Seven isolates displayed zone of clearance in starch hydrolysis test except Sample code 5, 7 and 8. Sample code 5, 7 and 8 isolates gave negative results for TSI test also. The isolates were subjected to amylase activity test. Maximum amylase was produced by Sample code 9 at 3.90 mg/ml after 24 hours of incubation. Sample code 10 showed least amylase production at 0.86 mg/ml.


Keywords: solubilisation, nitrogen fixation, PGPR, Bacillus sp. and Pseudomonas sp
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How to cite this article:

Shambhavi, Ravindra Kumar, Akash Tomar, Purushottam, Jitender Singh and Shishu Pal Singh. 2020. Isolation and Identification of Bacillus Species from Soil for Phosphate, Potassium Solubilisation and Amylase Production.Int.J.Curr.Microbiol.App.Sci. 9(5): 415-426. doi: https://doi.org/10.20546/ijcmas.2020.905.046