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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2019.8(9): 3041-3048
DOI: https://doi.org/10.20546/ijcmas.2019.809.348


A Prevalence Study of Uropathogenic Escherichia coli and Characterization of Virulence Markers
M.A. Ashiha Begum*, C.I. Indra Priyadasrshini and Sucila Anna
Tirunelveli Medical College, Tirunelveli – 627011, India
*Corresponding author
Abstract:

Urinary Tract Infections (UTI) account for more than 7 million visit for outpatient consultation.  UTI is the second commonest one, after the Respiratory Tract Infection. Prevalence in young women is 30 times more than in men. The prevalence of bacteriuria increases with hospitalization and concurrent diseases. UTI types of complicated, uncomplicated, nosocomial UTI, and especially Uropathogenic Escherichia coli (UPEC)  are some of important ones. The main aim of the study include to find the prevalence of uropathogenic E.coli Strains – UPEC at a Tertiary Care Centre; To Characterize the virulence factors of Uropathogens; To evaluate bacterial isolates contributing to the uropathogens, and its virulence factors; To study the antimicrobial susceptibility pattern of uropathogenic E.coli. A cross sectional & analytical study the conducted in a Tertiary Care centre involving Medical, Surgical, Nephrology, Urology, Paediatrics, Paediatrics Medicine, Paediatrics Surgery & Ob- Gyn, Outpatient departments. A Midstream  sample of urine collected and by routine methods the uropathogens were isolated, and UPEC’s virulence factors  determined and antibiogram for the same done. The study was undertaken  to find out UPEC  strains and virulence factors like Haemolysin,  Haemagglunation, Cell surface hydrophobicity, Serum resistance shown by Serum Bactericidal assay and Serotyping were detected. Among 320 urinary samples 138 UPEC identified  by the  above said virulence factors. Antibiogram done by Disc diffusion method in Muller Hinton agar by following NCCLS guidelines. This study showed incidence of UTI more in female, especially in postmenopausal women, Among children UTI more common in males. UPEC is one is the most important cause of UTI and its important virulence factors are haemolysin, haemagglutination, cell surface hydrophobicity and serum resistance were detected.


Keywords: UTI, Escherichia coli, Faecal flora, UPEC, Virulence markers
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How to cite this article:

Ashiha Begum, M.A., C.I. Indra Priyadasrshini and Sucila Anna. 2019. A Prevalence Study of Uropathogenic Escherichia coli and Characterization of Virulence Markers.Int.J.Curr.Microbiol.App.Sci. 8(9): 3041-3048. doi: https://doi.org/10.20546/ijcmas.2019.809.348