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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 3097-3104
DOI: https://doi.org/10.20546/ijcmas.2020.904.361


To Evaluate the Stability of Carbopol 934 based Transdermal Gel of Paracetamol Nanocapsules
Jayanti Mukherjee1*, Shivani Khoware1 and Sheetal Bhasin2
1Department of Pharmaceutical Chemistry, Shri Bherulal Pharmacy Institute, Indore, India 2Department of Microbiology, Maharaja Ranjit Singh College, Indore, India
*Corresponding author
Abstract:

The nanocapsules were prepared by the interfacial deposition technique proposed in the year 1989. The required quantity of the drug and benzyl benzoate were mixed and added dropwise in the organic phase containing the polymer PLGA dissolved in the two organic solvents. With continuous stirring at 4000rpm for 1 hour, soyalecithin was added in the above mixture. Finally, the later was added in the solution of Polaxamer 188 with a continuous stirring speed of 4000rpm for 30 minutes. Fine concentrated colloidal dispersion of paracetamol nanocapsules were obtained after the removal of organic solvents and high-speed centrifugation. Then anocapsules were characterized by scanning electron microscopy, zeta potential and particle size analysis. The compatibility of the excipients was analysed by FTIR. The nanogel of paracetamol was formulated with carbopol 934 and triethanolamine as the major ingredients. The transdermal formulation was tested for its in vitro permeation studies through the biomimetic dialysis membrane using Franz diffusion cell and entrapment efficiency was also conducted. The receiver solution was phosphate buffer (pH 7.4) and the permeability study was carried out for 12 hours. The transdermal gel was subjected to accelerated stability tests for 6 months and after this period microbiological tests were performed for common microorganisms. The qualitative and quantitative estimation of viable microorganisms present in the gel was evaluated which includes tests for total viable count (bacteria and fungi) and specified microbial organisms (Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa and Staphylococcus aureus). The accelerated stability study showed that the product is stable for the period of 6 months. The microbiological assay established the absence of common microorganisms. Transdermal gel formulation containing paracetamol can be prepared by the interfacial deposition technique using Carbopol 934 as a carrier. In vitro drug release data showed better physiochemical characteristics with no skin irritation reactions. Moreover, the accelerated stability test and the microbiological tests assured a stable product.


Keywords: Paracetamol nanocapsules, Accelerated Stability Test, Microbiological tests for organisms, Stability
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How to cite this article:

Jayanti Mukherjee, Shivani Khoware and Sheetal Bhasin. 2020. To Evaluate the Stability of Carbopol 934 based Transdermal Gel of Paracetamol Nanocapsules.Int.J.Curr.Microbiol.App.Sci. 9(4): 3097-3104. doi: https://doi.org/10.20546/ijcmas.2020.904.361