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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 2514-2524
DOI: https://doi.org/10.20546/ijcmas.2020.904.301


Detection of Beta Lactmase in Ampicilin Resistance Enterobacteriaceae Isolated from Human Stool in Ekiti State
K. Ekundayo* and A. K. Onifade
Department of Biology School of science College of Education Ikere Ekiti, EkitiState, Nigeria
*Corresponding author
Abstract:

This study was conducted to determine β-lactamase in ampicillin resistant members of the family Enterobacteriaceae isolated from human stool of apparently healthy human volunteer and sick humans’ stool from Federal Teaching Hospital Ido (FTH) and University Teaching Hospital Ado Ekiti in Ekiti State. Stools samples were collected and analyzed microbiologically using conventional techniques, antibiotics susceptibility testing by disc diffusion method and Double disc synergy test was used to determine β-lactamase producing bacterial isolates. The genetic basis of resistance was achieved by plasmid profiling and plasmid curing techniques. Four enteric bacteria, namely Escherichia coli, Klebsiella aerogenes, Salmonella typhi and Proteus vulgaris were isolated from the stool samples and majority of the bacterial isolate resistance is either plasmid or chromosomally mediated. The results showed that, extended spectrum β-lactamase (ESBL) producing bacteria can be found in apparently healthy human volunteer stool and major tertiary hospitals in the state. Therefore personal hygiene in our local canteen and eateries, proper hand washing at individual homes, proper laboratory diagnosis for any infection, appropriate prescription of drugs should be considered, infectious disease control of every hospital should be effective and the use of antibiotics in poultry farm should be regulated in Ekiti State as this will help to monitor the trend of ESBLs infection.


Keywords: β-lactamase,Enterobacteriaceae,Stools samples
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How to cite this article:

Ekundayo, O. K. and Onifade, A. K. 2020. Detection of Beta Lactmase in Ampicilin Resistance Enterobacteriaceae Isolated from Human Stool in Ekiti State.Int.J.Curr.Microbiol.App.Sci. 9(4): 2514-2524. doi: https://doi.org/10.20546/ijcmas.2020.904.301