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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 2400-2405
DOI: https://doi.org/10.20546/ijcmas.2020.904.288


Development of Loop Mediated Isothermal Amplification (LAMP) Assay for Detection of PCV2
J. B. Rajesh1*, Swaraj Rajkhowa2, Umesh Dimri3, Hridayesh Prasad1, Kalyan Sarma1, G. E. Chethan1, Parthasarathi Behera4 and Manjisa Choudhury2
1Department of Veterinary Medicine, College of veterinary Sciences and Animal Husbandry (Central Agricultural University), Selesih, Aizawl, Mizoram, India: 796015
2Indian Council for Agricultural Research-National Research Centre on Pig (ICAR-NRC on Pig), Rani, Guwahati, Assam, India: 781131
3Division of Medicine, Indian Council for Agricultural Research- Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Bareilly, Uttar Pradesh, India: 243122
4Department of Physiology and Biochemistry, College of veterinary Sciences and Animal Husbandry (Central Agricultural University), Selesih, Aizawl, Mizoram, India: 796015
*Corresponding author
Abstract:

Porcine circovirus2 (PCV2) is an emerging pathogen of porcine farming. PCV2 is a non-enveloped circular single-stranded DNA virus. PCV2 infection produces variety clinical symptoms and thus causes huge economic loss. Various diagnostic methods are available for the diagnosis of PCV2 infection. However, there is still no rapid, sensitive and practical method for detecting PCV2. Loop mediated isothermal amplification (LAMP) has been used to detect a variety of pathogenic microorganisms. In this study, we developed a LAMP for detection of PCV2. Primers used for LAMP were designed using conserved region of PCV2 sequences available in Genbank. The LAMP assay was developed by standard protocol including determination sensitivity and specificity of the assay. The LAMP assay provides a rapid, sensitive, reliable way to detect PCV2 and can be used for the epidemiological investigation of PCV2.


Keywords: Diagnosis, LAMP, PCR, PCV2, Porcine
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How to cite this article:

Rajesh,. J. B., Swaraj Rajkhowa, Umesh Dimri, Hridayesh Prasad, Kalyan Sarma, G. E. Chethan, Parthasarathi Behera and Manjisa Choudhury. 2020. Development of Loop Mediated Isothermal Amplification (LAMP) Assay for Detection of PCV2.Int.J.Curr.Microbiol.App.Sci. 9(4): 2400-2405. doi: https://doi.org/10.20546/ijcmas.2020.904.288