National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The Groundnut bud necrosis virus (GBNV) induced symptoms such as bud necrosis, chlorotic and necrotic spots on leaves of tomato, chilli and brinjal were collected from field for mechanical inoculation on cow pea (var. C-152) as local lesion host. The electron microscopic observation of the purified preparation of diseased samples showed the presence of spherical, membrane bound particles (80-120 nm) of typical tospoviruses. Host range study was conducted to GBNV in which Arachis hyopogaea, Vigna unguiculata, Phaseolus vulgaris, Dolichos lablab, Glycine max from Leguminaceae, Cucurbita maxima, Citrullus lanatus from Cucurbitaceae, Gomphrena globasa from Amaranthaceae, four Nicotiana sp. Petunia hybrid, Datura stramonium, D. metal from Solanaceae, Chenopodium quinoa, C. amaranticolor from Chenopodiaceae, Tagetes spp. from Asteraceae, Jasminum sp. from Oleaceae, Vinca rosea from Apocyanaceae, Impatiens sp. from Balsominaceae and some weeds – Solanum nigrum, Physalis minima, Portulaca oleracea, Trianthema portulacastrum showed the local and systemic infection which was similar to GBNV symptoms. For further confirmation DAC-ELISA was performed to all infected plants including tomato, chilli and brinjal samples which showed positive reaction to the GBNV specific antisera. The RT-PCR was performed after total RNA extraction from infected leaves of Solanaceous vegetable crops. A fragment of approximately 0.8kb corresponding to the N gene of S RNA was amplified, cloned and sequenced. The nucleotide sequence of N gene from the infected Solanaceous vegetable crops showed highest identities of 93% to 99% nucleotide with other GBNV isolates of black gram (AY512650.1), tomato (AY463968.1), pea (JF281101), chilli (AY618567.1), Solanum nigrum (KX244339.1), (KX244334.1), groundnut (JX198661.1) and potato (AF515821.1).
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