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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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Original Research Articles                      Volume : 9, Issue:4, April, 2020

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1611-1616
DOI: https://doi.org/10.20546/ijcmas.2020.904.188


Detection of Bovine Papillomavirus in Cutaneous Lesions by Polymerase Chain Reaction (PCR) in Cattle
Lurthu Reetha*, R. Manickam and B. Puvarajan
Department of Veterinary Microbiology, Veterinary College and Research Institute, Orathanadu, Thanjavur, India
*Corresponding author
Abstract:

Bovine papillomavirus (BPV) causes benign tumours in the mucosal and cutaneous epithelium and is characterized by the presence of warts. The present study includes the molecular identification of BPV strains in samples of warts using degenerate polymerase chain reaction (PCR) primers FAP59/64. Wart samples were collected from the cattle having typical lesions on various parts of the body. The present study showed that PCR amplification with the primers FAP59/64, which partially amplify the L1 gene and showed the 470bp amplicon size, indicating BPV. The results in this study are important for the development of prophylactic and therapeutic measures that contribute to reducing the economic losses associated with BPV.


Keywords: Livestock, Bovine papilloma, Virology, Polymerase chain reaction

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How to cite this article:

Lurthu Reetha, T., R. Manickam and Puvarajan, B. 2020. Detection of Bovine Papillomavirus in Cutaneous Lesions by Polymerase Chain Reaction (PCR) in Cattle.Int.J.Curr.Microbiol.App.Sci. 9(4): 1611-1616. doi: https://doi.org/10.20546/ijcmas.2020.904.188
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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