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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1996-1999
DOI: https://doi.org/10.20546/ijcmas.2020.904.239


Single Nucleotide Polymorphism C-2161G in Promoter Region of Prolactin Gene in ‘N’ Strain of White Leghorn
Azhaguraja Manoharan1*, S. Sankaralingam1, P. Anitha1, Binoj Chacko1 and T. V. Aravindakshan2
1Department of Poultry Science, College of Veterinary and Animal Sciences,Mannuthy, Thrissur -680 651, Kerala, India
2Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Mannuthy, Thrissur -680 651, Kerala, India
*Corresponding author
Abstract:

Single nucleotide polymorphisms are the most commonly observed genetic variation among particular population, generally known as SNPs (pronounced “snips”). The main objective of the current research was recognition of SNP C-2161G in promoter region of prolactin gene in ‘N’ strain of White Leghorn and its interrelation with production traits. A total of 200 birds of ‘N’ strain of White Leghorn were randomly selected from All India Co-ordinated Research Project (AICRP) on Poultry Improvement, Mannuthy, Thrissur, Kerala. Blood samples were collected from the randomly selected birds and isolation of genomic DNA was done using DNA isolation kit. PCR and PCR-RFLP analysis was accomplished to detect a SNP at C-2161G site in promoter region of prolactin gene. The genotypes are represented as CC, CG and GG for C-2161G. All the 200 birds were noticed with same genotype (CC) and production performance.


Keywords: Prolactin, SNP, PCR-RFLP, White Leghorn, Genotype
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How to cite this article:

Azhaguraja Manoharan, S. Sankaralingam, P. Anitha, Binoj Chacko and Aravindakshan, T. V. 2020. Single Nucleotide Polymorphism C-2161G in Promoter Region of Prolactin Gene in ‘N’ Strain of White Leghorn.Int.J.Curr.Microbiol.App.Sci. 9(4): 1996-1999. doi: https://doi.org/10.20546/ijcmas.2020.904.239