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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2016: 92.3
Index Copernicus ICI Journals Master List 2016 - IJCMAS--ICV 2016: 92.3
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 761-768
DOI: https://doi.org/10.20546/ijcmas.2017.603.088


A New Method Development and Validation for Identification and Quantification of Methoxyfenozide Insecticide Residues in Grapes using High Performance Liquid Chromatography
S.N.V.S. Murthy1, Tentu Nageswara Rao2*, Prathipati Revathi2 and S. Seshamma3
1Department of Organic Chemistry, D.L.R. P.G. College, Gollalamamidada, AP, India
2Department of Chemistry, Krishna University, Machilipatnam, AP India
3Department of Bio Chemistry, AN University, Guntur, AP, India
*Corresponding author
Abstract:

A simple and inexpensive method was developed using liquid - liquid extraction, together with high performance chromatographic method for determination of methoxyfenozide residues in grapes. The evaluated parameters include the extracts by distilled water and acetonitrile solvents. The method was validated using grapes samples spiked with methoxyfenozide at different fortification levels (0.05, 0.1 and 0.5 µg/g). Average recoveries (using each concentration three replicates) ranged 86-95 %, with relative standard deviations less than 5%, calibration solutions concentration in the range 0.05-5.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.02µg/g and 0.05µg/g respectively. Finally the grapes residue samples were analyzed by HPLC.


Keywords: HPLC, methoxyfenozide, Grapes, LOD and LOQ.
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How to cite this article:

Murthy, S.N.V.S., Tentu Nageswara Rao, Prathipati Revathi and Seshamma, S. 2017. A New Method Development and Validation for Identification and Quantification of Methoxyfenozide Insecticide Residues in Grapes using High Performance Liquid Chromatography.Int.J.Curr.Microbiol.App.Sci. 6(3): 761-768. doi: https://doi.org/10.20546/ijcmas.2017.603.088