National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
RNA interference (RNAi) is a powerful molecular tool for gene discovery and functional validation for candidate vaccine antigens from the vast amount of information available within EST datasets. In this paper we report the knock down in the phenotypic expression of Hemonchus contortus astacin-like MTP gene associated with reproduction and blood-feeding of this parasite which is predominantly expressed in late larval and adult stages by RNAi interference. The RNAi experiments were conducted utilizing the soaking protocol. Results showed that there was interference in expression of each H. contortus astacin 3' or 5'ends transcripts individually as judged by RT-PCR’s. The resultant dsRNA products were also found to be gene-specific. However no visible phenotypes were detected after simultaneous RNAi treatments for both H. contortus astacin 3'and 5'end gene transcripts.
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