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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1175-1184
DOI: https://doi.org/10.20546/ijcmas.2020.904.139


RNA Interference of the Hemonchus contortus Astacin-like MTP Gene by the Soaking Method
Charles Muleke Inyagwa1* and Dave Knox2
1Faculty of Veterinary Medicine & Surgery, Department of Veterinary Pathology, Microbiology & Parasitology; Egerton University, P.O. Box 536-20115, Egerton, Kenya
2Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik, EH26 0PZ, UK
*Corresponding author
Abstract:

RNA interference (RNAi) is a powerful molecular tool for gene discovery and functional validation for candidate vaccine antigens from the vast amount of information available within EST datasets. In this paper we report the knock down in the phenotypic expression of Hemonchus contortus astacin-like MTP gene associated with reproduction and blood-feeding of this parasite which is predominantly expressed in late larval and adult stages by RNAi interference. The RNAi experiments were conducted utilizing the soaking protocol. Results showed that there was interference in expression of each H. contortus astacin 3' or 5'ends transcripts individually as judged by RT-PCR’s. The resultant dsRNA products were also found to be gene-specific. However no visible phenotypes were detected after simultaneous RNAi treatments for both H. contortus astacin 3'and 5'end gene transcripts.


Keywords: RNA interference, H. contortus, Astacin-like MTP gene, Soaking method
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How to cite this article:

Charles Muleke Inyagwa and Dave Knox. 2020. RNA Interference of the Hemonchus Contortus Astacin- like MTP Gene by the Soaking Method.Int.J.Curr.Microbiol.App.Sci. 9(4): 1175-1184. doi: https://doi.org/10.20546/ijcmas.2020.904.139