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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1154-1159
DOI: https://doi.org/10.20546/ijcmas.2020.904.136


B-Cell Linear Epitope Prediction on VP2 Partial Coat Protein Predicted Model of CPV-2b Isolate using Bioinformatics
Shalini Sharma1*, Praseenjit Dhar1, Inderjeet Singh Yadav2 and Mandeep Sharma1
1Department of Veterinary Microbiology, College of Veterinary and Animal sciences, CSKHPKV Palampur, Himachal Pradesh, India
2School of Animal Biotechnology, PAU, Ludhiana, Punjab, India
*Corresponding author
Abstract:

Canine parvovirus is the major cause of haemorrhagic gastroenteritis in canines worldwide. There is a high morbidity and mortality in the young puppies. The currently available CPV vaccine is protecting from all antigenic strains but still there is vaccination failure as the strains are emerging at higher rate. The VP2 coat protein is the major coat protein it contains many B-cell epitopes against which many neutralizing antibodies can form, this structural protein of parvovirus can produce virus-like particles (VLPs) by a self-assembly process in vitro, making VLPs attractive vaccine candidates. So in this study a predicted protein model of partial protein coat of canine parvovirus isolate obtained after PCR detection and cell culture adaptation is made along with the display of B cell linear epitopes using bioinformatics tools. The protein sequence is deduced from the nucleotide sequence of the isolate obtained after sequencing. The length of nucleotide sequence of partial coat protein of CPV-2b isolate was 424 bp long the translated protein was 125 amino acid long and the B-Cell linear epitopes lies between the 59-75 amino acid positions. So in conclusion B-Cell epitope prediction on 3D model of CPV-2b isolate will be helpful in the vaccine design and for the production of therapaeutic antibodies moreover immunoinformatics is time saving does not requires extensive labour and animal experimentation.


Keywords: Epitopes, CPV-2b and PDB
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How to cite this article:

Shalini Sharma, Praseenjit Dhar, Inderjeet Singh Yadav and Mandeep Sharma. 2020. B-Cell Linear Epitope Prediction on VP2 Partial Coat Protein Predicted Model of CPV-2b Isolate using Bioinformatics.Int.J.Curr.Microbiol.App.Sci. 9(4): 1154-1159. doi: https://doi.org/10.20546/ijcmas.2020.904.136