National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Non-symbiotic (Azotobacter) nitrogen obsession is a critical wellspring of settled nitrogen for the biosphere. Microorganisms catalyze natural nitrogen obsession with the chemical nitrogenase, which has been exceedingly moderated through development. Aside from settling nitrogen Azotobacter can be utilized as a plant development advancing rhizobacteria (PGPR). Soil tests were gathered from differing agrarian documented of Misrod (District Raisen) Bhopal (Madhya Pradesh). The dirt example were chosen from Rhizospheric locale of Cicer arietinum, speak to an extensive variety of physical and compound properties, 16S rDNA based PCR Amplification method were utilized for quality articulation. Confined protein (Alu I,) favored for RFLP and RAPD (OPK 20) 5’-GTGTCGCGAG-3’primer for fingerprinting and gives a high level of segregation between Azotobacter species. Phylogenetic examination16S-rDNA based PCR and elucidation in the wake of anticipating dendrogram changes of Azotobacter strains acquired affirms varieties among species. Presence of variable game plan on genome by reflecting slight variety coming about into foundation of decent variety among strain.
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