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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2018 - IJCMAS--ICV 2018: 95.39 For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 1103-1115
DOI: https://doi.org/10.20546/ijcmas.2020.904.131


Microbial Diversity among Azotobacter strains and Efficacy Test on Chickpea (Cicer arietinum)
Jitendra Malviya1* and Bhupendra Prasad2
1Department of Biotechnology and Bioinformatic centre,Barkatullah University, Bhopal, India
2Department of Microbiology, Career College, Bhopal, India
*Corresponding author
Abstract:

Non-symbiotic (Azotobacter) nitrogen obsession is a critical wellspring of settled nitrogen for the biosphere. Microorganisms catalyze natural nitrogen obsession with the chemical nitrogenase, which has been exceedingly moderated through development. Aside from settling nitrogen Azotobacter can be utilized as a plant development advancing rhizobacteria (PGPR). Soil tests were gathered from differing agrarian documented of Misrod (District Raisen) Bhopal (Madhya Pradesh). The dirt example were chosen from Rhizospheric locale of Cicer arietinum, speak to an extensive variety of physical and compound properties, 16S rDNA based PCR Amplification method were utilized for quality articulation. Confined protein (Alu I,) favored for RFLP and RAPD (OPK 20) 5’-GTGTCGCGAG-3’primer for fingerprinting and gives a high level of segregation between Azotobacter species. Phylogenetic examination16S-rDNA based PCR and elucidation in the wake of anticipating dendrogram changes of Azotobacter strains acquired affirms varieties among species. Presence of variable game plan on genome by reflecting slight variety coming about into foundation of decent variety among strain.


Keywords: Azotobacter, nitrogenase, PGPR, PCR, 16S-rDNA, Phylogenetic analysis, Dendrogram
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How to cite this article:

Jitendra Malviya and Bhupendra Prasad. 2020. Microbial Diversity among Azotobacter Strains and Efficacy Test on Chickpea (Cicer arietinum).Int.J.Curr.Microbiol.App.Sci. 9(4): 1103-1115. doi: https://doi.org/10.20546/ijcmas.2020.904.131