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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2019)
[Effective from January 1, 2019]
For more details click here

ICV 2018: 95.39
Index Copernicus ICI Journals Master List 2017 - IJCMAS--ICV 2018: 95.39
For more details click here

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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2020.9(4): 997-1004
DOI: https://doi.org/10.20546/ijcmas.2020.904.118


Screening of Antifungal Activity of Pleurotus pulmonarius, Pleurotus florida and Shizophyllum commune
Vinita Dahima1, A. Doshi1 and Hanuman Singh2*
1Department of Plant Pathology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur 313 001, Rajasthan, India
2College of Horticulture & Forestry, Agriculture University, Kota, 324001, Rajasthan, India
*Corresponding author
Abstract:

Antifungal activities of the three mushrooms Pleurotus pulmonarius, Pleurotus florida and Schizophyllum commune ethanol extracts were evaluated against some plant pathogenic fungi (Aspergillus flavus, Aspergillus niger and Alternaria solani, Colletotrichum graminicola, Fusarium oxysporum) by using agar well diffusion method. Minimum inhibitory concentration (MIC) was investigated for 1-20 mg/ml concentration to find out the lowest concentration of the sample that inhibits the growth of the test organisms. The standard agar dilution protocol with doubling dilution was used. The result revealed that the three mushrooms extract used in the present investigation possessed varying degrees of antifungal activities against the test plant pathogenic fungi. Ethanolic extract of Pleurotus florida showed highest inhibition activity against Fusarium oxysporu, and S. commune showed maximum zone of inhibition (ZOI) against A. niger, while, P. pulmonarius showed maximum zone of inhibition (ZOI) against A. solani. The minimum inhibitory concentrations (MIC) ranged between 9.67 and18.98 mg/ml for fungi, the lowest MIC, the extract was still be effective because of the presence of bio-active compounds. The findings revealed that the mushrooms extracts tested in the current work contains potential therapeutic compounds against some of the economically important plant diseases caused by bacteria and fungi.


Keywords: Antifungal, Mushrooms, Fusarium oxysporum, Minimum inhibitory concentrations, Agar well diffusion
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How to cite this article:

Vinita Dahima, A. Doshi and Hanuman Singh. 2020. Screening of Antifungal Activity of Pleurotus pulmonarius, Pleurotus florida and Shizophyllum commune.Int.J.Curr.Microbiol.App.Sci. 9(4): 997-1004. doi: https://doi.org/10.20546/ijcmas.2020.904.118